Abstract
A gene encoding an endo-β-1,3-glucanase from Thermotoga maritima MSB8 (TmβG) was cloned and expressed in Escherichia coli. The purified enzyme produced various β-1,3-glucooligosaccharides from soluble laminarin, and mainly β-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80°C, respectively. TmβG inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.
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Woo, CB., Kang, HN. & Lee, SB. Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima . Food Sci Biotechnol 23, 1243–1246 (2014). https://doi.org/10.1007/s10068-014-0170-9
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DOI: https://doi.org/10.1007/s10068-014-0170-9