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Cytotoxicity of the white ginseng extract and red ginseng extract treated with partially purified β-glucosidase from Aspergillus usamii KCTC 6954

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Abstract

Red ginseng extract (RGE) and white ginseng extract (WGE) were treated with partially purified β-glucosidase to increase a production of minor ginsenosides. The enzyme produced from Aspergillus usamii KCTC 6954 was precipitated with (NH4)2SO4. Ginseng extracts were treated with a crude extract possessing β-glucosidase activity (1,089.2 μM/mL·min) at 60°C for 72 h. The results of HPLC showed that enzyme-treated RGE and enzymetreated WGE have increased amounts of minor ginsenosides compared to each controls implying that the ginsenoside Rb1 in WGE and RGE is converted enzymatically to Rd, F2, Rg3, and compound K. In cytotoxicity study, 2.5 mg/mL of RGE, 1.25 mg/mL of ERGE, and 5 mg/mL of WGE and EWGE were effective against the HepG2, AGS, and DLD-1, but HeLa and SK-MES-1 were not affected at any concentration. The results suggested that cytotoxicity of ginseng extracts treated with β-glucosidase were greater than that of each control against cancer cells.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 143-701, Korea

    Mina Jo, Ji Eun Jung, Jae Hoon Lee, Hyun Joo Yoon & Hyun-Dong Paik

  2. Dongwon Korea Ginseng Co., Ltd., Seoul, 365-800, Korea

    Se-Ho Park

  3. Bio/Molecular Informatics Center, Konkuk University, Seoul, 143-701, Korea

    Kee-Tae Kim & Hyun-Dong Paik

Authors
  1. Mina Jo
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  2. Ji Eun Jung
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  3. Jae Hoon Lee
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  5. Hyun Joo Yoon
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Correspondence to Hyun-Dong Paik.

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Jo, M., Jung, J.E., Lee, J.H. et al. Cytotoxicity of the white ginseng extract and red ginseng extract treated with partially purified β-glucosidase from Aspergillus usamii KCTC 6954. Food Sci Biotechnol 23, 215–219 (2014). https://doi.org/10.1007/s10068-014-0029-0

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  • DOI: https://doi.org/10.1007/s10068-014-0029-0

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