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Inhibitory effects of chestnut inner skin extracts on melanogenesis

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Abstract

Antioxidant capacities (ABTS radical scavenging assay and ferric reducing/antioxidant power assay), mushroom tyrosinase inhibitory effect, and α-melanocyte stimulating hormone (α-MSH) induced melanogenesis inhibitory effect of 60% methanol extracts and ethylacetate fractions from chestnut inner skin in B16F10 cells were investigated to inspect whitening effect. Above research showed that 60% methanol extracts and ethylacetate fractions from chestnut inner skin resulted in a dose-dependent manner on in vitro antioxidant effects. Especially, the ethylacetate fractions inhibited enzyme activity of mushroom tyrosinases with an IC50 value of 160 μg/mL. Ethylacetate fractions from chestnut inner skin also decreased cellular melanin synthesis in B16F10 melanoma cells. Expression of tyrosinase showed that ethylacetate fractions from chestnut inner skin significantly decreased cellular melanogenesis. Consequently, these results suggest that chestnut inner skin extracts can be considered for a whitening agent of human skin.

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Authors and Affiliations

  1. Division of Applied Life Science (BK21), Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam, 660-701, Korea

    Ji Hye Kim, Gwi Nam Choi, Ji Hyun Kwak, Hee Rok Jeong & Ho Jin Heo

  2. Jeollanamdo Institute of Natural Resources Research (JINR), Jangheung, Jeonnam, 529-851, Korea

    Ji Hye Kim

  3. Wooyang Frozen Food Co., Ltd., Seocheon, Chungnam, 325-907, Korea

    Chang-Ho Jeong

  4. Department of Speicial Purpose Trees, Korea Forest Research Institute, Suwon, Gyeonggi, 441-847, Korea

    Uk Lee & Man-Jo Kim

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  1. Ji Hye Kim
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  2. Gwi Nam Choi
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  3. Ji Hyun Kwak
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  4. Chang-Ho Jeong
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  8. Ho Jin Heo
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Correspondence to Ho Jin Heo.

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Kim, J.H., Choi, G.N., Kwak, J.H. et al. Inhibitory effects of chestnut inner skin extracts on melanogenesis. Food Sci Biotechnol 21, 1571–1576 (2012). https://doi.org/10.1007/s10068-012-0209-8

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  • DOI: https://doi.org/10.1007/s10068-012-0209-8

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