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Correlation between antioxidant concentrations and activities of Yuja (Citrus junos Sieb ex Tanaka) and other citrus fruit

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Abstract

The antioxidant concentrations and activities of tangerines, yuja, lemons, and oranges in the Korean marketplace have been investigated. Flesh and peel tissues of the fruit were analyzed. The total vitamin C, total phenolics, flavonoid, hesperidin, and total antioxidant activity of all citrus types were higher in peel than flesh tissues. Total phenolic concentrations of flesh tissues were in the following order: lemon>yuja>tangerine>orange, while those concentrations in the peel were tangerine >yuja>orange>lemon. Lemon and yuja had higher hesperidin concentrations in flesh and peel tissues, respectively. Among the flesh samples, the total flavonoid and total phenolic concentrations were highly related with total antioxidant activity. The relationship between flavonoid and hesperidin was also strong among flesh and peel tissues. Our results indicate that citrus fruit includes high antioxidant compounds and activity and that citrus peel could be a good source of food ingredients.

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References

  1. Simonne AH, Ritenour MA. Citrus [orange, lemon, mandarin, grapefruit, lime, and other citrus fruits]. pp. 90–117. In: Health-Promoting Properties of Fruit & Vegetables. Terry L (ed). CABI, Cambridge, MA, USA (2011)

    Chapter  Google Scholar 

  2. Guthrie N, Morley K, Hasegawa S, Manners GD, Vandenberg T. Inhibition of human breast cancer cells by citrus limonoids. pp.164–174. In: Citrus Limonoids: Functional Chemicals in Agriculture and Foods. Berhow MA, Hasegawa S, Manners GD (eds). ACS Symposium Series 758; American Chemical Society, Washington, DC, USA (2000)

    Chapter  Google Scholar 

  3. Tian QG, Miller EG, Ahmad H, Tang L, Patil BS. Differential inhibition of human cancer cells proliferation by citrus limonoids. Nutr. Cancer 40: 180–184 (2001)

    Article  CAS  Google Scholar 

  4. Benavente-Garcia O, Castillo J, Marin FR, Ortuno A, Del Rio J. Uses and properties of Citrus flavonoids. J. Agr. Food Chem. 45: 4505–4515 (1997)

    Article  CAS  Google Scholar 

  5. Benavente-Garcia O, Castillo J. Update on uses and properties of citrus flavonoids: New findings in anticancer, cardiovascular, and anti-inflammatory activity. J. Agr. Food Chem. 56: 6185–6205 (2008)

    Article  CAS  Google Scholar 

  6. Tundis R, Loizzo MR, Bonesi M, Menichini F, Mastellone V, Colica C, Menichini F. Comparative study on the antioxidant capacity and cholinesterase inhibitory activity of Citrus aurantifolia Swingle, C. aurantium L., and C. bergamia Risso and Poit. Peel essential oils. J. Food Sci. 77: 40–46 (2012)

    Article  Google Scholar 

  7. Heo HJ, Kim DO, Shin SC, Kim MJ, Kim BG, Shin DH. Effect of antioxidant flavanone, naringenin, from Citrus junos on neuroprotection. J. Agr. Food Chem. 52: 1520–1525 (2004)

    Article  CAS  Google Scholar 

  8. Monforte MT, Trovato A, Kirjavainen S, Forestier AM, Galati EM, Lo Curto RB. Biological effects of hesperidin, a Citrus flavonoid (note II): Hypolipidemic activity on experimental hypercholesterolemia in rat. Pharmacology 50: 595–599 (1995)

    CAS  Google Scholar 

  9. Ko KC, Kim CS, Lee NH, Lee SP, Moon DK. Determination of β-cryptoxanthin in peel and flesh of citrus fruits produced in Jeju Island. Food Sci. Biotechnol. 9: 288–291 (2000)

    Google Scholar 

  10. Kim DH, Kim DG, Lee DY, Kim KE, Kim CW. Physicochemical characterization of pectin extracted from Jeju mandarin (Citrus unshiu) peels with citric acid. Food Sci. Biotechnol. 9: 95–98 (2000)

    Google Scholar 

  11. Kim YC, Koh KS, Koh JS. Changes of flavonoids in the peel of Jeju native citrus fruits during maturation. Food Sci. Biotechnol. 10: 483–487 (2001)

    Google Scholar 

  12. Kang SH, Lee YJ, Lee CH, Kim SJ, Lee DH, Lee YK, Park DB. Physiological activities of peel of Jeju-indigenous Citrus sunki Hort. Tanaka. Korean J. Food Sci. Technol. 37: 983–988 (2005)

    Google Scholar 

  13. Lim HK, Yoo ES, Moon JY, Jeon YJ, Cho SM. Antioxidant activity of extracts from dangyuja (Citrus grandis Osbeck) fruits produced in Jeju Island. Food Sci. Biotechnol. 15: 312–316 (2006)

    Google Scholar 

  14. Yoo KM, Hwang IK. In vitro effect of yuja (Citrus junos Sieb ex Tanaka) extracts on proliferation of human prostate cancer cells and antioxidant activity. Korean J. Food Sci. Technol. 36: 339–344 (2004)

    Google Scholar 

  15. Yoo KM, Hwang IK, Park JH, Moon B. Major phytochemical composition of 3 native Korean citrus varieties and bioactive activity on V79-4 cells induced by oxidative stress. J. Food Sci. 74: 462–468 (2009)

    Article  Google Scholar 

  16. Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M. Quantitation of flavonoid constituents in citrus fruits. J. Agr. Food Chem. 47: 3565–3571 (1999)

    Article  CAS  Google Scholar 

  17. Yoo KM, Lee KW, Park JB, Lee HJ, Hwang IK. Variation in major antioxidants and total antioxidant activity of yuja (Citrus junos Sieb ex Tanaka) during maturation and between cultivars. J. Agr. Food Chem. 52: 5907–5913 (2004)

    Article  CAS  Google Scholar 

  18. Sawamura M, Sun SH, Ozaki K, Ishikawa J, Ukeda H. Inhibitory effects of citrus essential oils and their components on the formation of N-nitrosodimethylamine. J. Agr. Food Chem. 47: 4868–4872 (1999)

    Article  CAS  Google Scholar 

  19. Choi HS, Song HS, Ukeda H, Sawamura M. Radical scavenging activities of citrus essential oils and their components: Detection using 1,1-diphenyl-2-picrylhydrazyl. J. Agr. Food Chem. 48: 4156–4161 (2000)

    Article  CAS  Google Scholar 

  20. Liu M, Li XQ, Weber C, Lee CY, Brown J, Liu RH. Antioxidant and antiproliferative activities of raspberries. J. Agr. Food Chem. 51: 2926–2930 (2002)

    Article  Google Scholar 

  21. Meyers KJ, Watkins CB, Pritts MP, Liu RH. Antioxidant and antiproliferative activities of strawberries. J. Agr. Food Chem. 51: 6887–6892 (2003)

    Article  CAS  Google Scholar 

  22. Shin Y, Ryu JA, Liu RH, Nock JF, Watkins CB. Harvest maturity, storage temperature, and relative humidity affect fruit quality, antioxidant contents and activity, and inhibition of cell proliferation of strawberry fruit. Postharvest Biol. Tec. 49: 201–209 (2008)

    Article  CAS  Google Scholar 

  23. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method. Enzymol. 299: 152–178 (1999)

    Article  CAS  Google Scholar 

  24. Belajová E, Suhaj M. Determination of phenolic constituents in citrus juices: Method of high performance liquid chromatography. Food Chem. 86: 339–343 (2004)

    Article  Google Scholar 

  25. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28: 5–30 (1995)

    Article  Google Scholar 

  26. Kim DO, Lee KW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J. Agr. Food Chem. 50: 3713–3717 (2002)

    Article  CAS  Google Scholar 

  27. Peterson JJ, Beecher GR, Bhagwat SA, Dwyer JT, Gebhardt SE, Haytowitz DB, Holden JM. Flavonones in grapefruit, lemon, and lime: A compilation and review of the data from the analytical literature. J. Food Compos. Anal. 19: 74–80 (2006)

    Article  Google Scholar 

  28. Hasegawa S, Maier VP. Some aspects of citrus biochemistry and juice quality. Proc. Int. Soc. Citric. 2: 914–918 (1981)

    CAS  Google Scholar 

  29. Outuno A, Reynaldo I, Fuster MD, Botia J, Puig DG, Sabater F, Lido’n AG, Porras I, Del Rio JA. Citrus cultivars with high flavonoids contents in the fruits. Sci. Hortic.-Amsterdam 68: 231–236 (1997)

    Article  Google Scholar 

  30. Eun JB, Jung YM, Woo GJ. Identification and determination of dietary fibers and flavonoids in pulp and peel of Korean tangerine (Citrus aurantium). Korean J. Food Sci. Technol. 28: 371–377 (1996)

    Google Scholar 

  31. Robards K, Antolovich MA. Critical review: Analytical chemistry of fruit bioflavonoids. Analyst 122: 11–34 (1997)

    Article  Google Scholar 

  32. Manthey JA, Guthrie N. Antiproliferative activities of citrus flavonoids against six human cancer cell lines. J. Agr. Food Chem. 50: 5837–5843 (2002)

    Article  CAS  Google Scholar 

  33. Kanaze FI, Gabrieli C, Kokkalou E, Georgarakis M, Niopas I. Simultaneous reversed-phase high-performance liquid chromatographic method for the determination of diosmin, hesperidin and naringin in different citrus fruit juices and pharmaceutical formulations. J. Pharm. Biomed. Anal. 33: 243–249 (2003)

    Article  CAS  Google Scholar 

  34. Gorinstein S, Martin-Belloso O, Park YS, Haruenkit R, Lojek A, Ciz M, Caspi A, Libman I, Trakhtenberg S. Comparison of some biochemical characteristics of different citrus fruits. Food Chem. 74: 309–315 (2001)

    Article  CAS  Google Scholar 

  35. Sun J, Chu YF, Wu XZ, Liu RH. Antioxidant and anti proliferative activities of common fruits. J. Agr. Food Chem. 50: 7449–7454 (2002)

    Article  CAS  Google Scholar 

  36. Rekika D, Khanizadeh S, Deschenes M, Levasseur A, Charles MT, Tsao R, Yang R. Antioxidant capacity and phenolic content of selected strawberry genotypes. Hortscience 40: 1777–1781 (2005)

    CAS  Google Scholar 

  37. Wang H, Cao GH, Prior RL. Total antioxidant capacity of fruits. J. Agr. Food Chem. 44: 701–705 (1996)

    Article  CAS  Google Scholar 

  38. Vinson JA, Su XH, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: Fruits. J. Agr. Food Chem. 49: 5315–5321 (2001)

    Article  CAS  Google Scholar 

  39. Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr. 78: 517–520 (2003)

    Google Scholar 

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Correspondence to Youngjae Shin.

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Shin, Y. Correlation between antioxidant concentrations and activities of Yuja (Citrus junos Sieb ex Tanaka) and other citrus fruit. Food Sci Biotechnol 21, 1477–1482 (2012). https://doi.org/10.1007/s10068-012-0195-x

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