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Detection of feline calicivirus as norovirus surrogate in food and water sources using filtration and real-time RT-PCR

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Abstract

Norovirus (NoV) is a major cause of acute non-bacterial gastroenteritis in all age groups worldwide. To detect NoV from foods, polyethylene glycol (PEG) precipitation or ultracentrifugation methods are generally used with reverse transcription (RT)-PCR. These methods need to use complicated procedures and varied buffers depending on the kinds of food matrices. In this study, we suggested a universal method to recover NoV in food and water samples as a prior step to real-time RT-PCR. As a NoV surrogate model, feline calicivirus (FCV) was used. FCV was artificially inoculated to samples, and then concentrated by the adsorption-elution method using negatively charged membrane filters. The detection limit was 4.3×101 PFU/250 mL for distilled water, 4.3×102 PFU/250 mL for environmental waters, and 4.3×102 PFU/15 g for lettuce and oyster. We were able to identify the possibility of one universal and time-saving method to detect NoV in food and water samples without modifications.

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Authors and Affiliations

  1. Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk, 361-763, Korea

    Min-Gyu Cho, Hye-Mi Jeong & Kwang-Yup Kim

  2. Ahn Department of Food Service Industry, Seowon University, Cheongju, Chungbuk, 361-742, Korea

    Jun-Bae Ahn

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  1. Min-Gyu Cho
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  2. Hye-Mi Jeong
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  3. Jun-Bae Ahn
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  4. Kwang-Yup Kim
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Correspondence to Kwang-Yup Kim.

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Cho, MG., Jeong, HM., Ahn, JB. et al. Detection of feline calicivirus as norovirus surrogate in food and water sources using filtration and real-time RT-PCR. Food Sci Biotechnol 20, 1475–1480 (2011). https://doi.org/10.1007/s10068-011-0204-5

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  • DOI: https://doi.org/10.1007/s10068-011-0204-5

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