Skip to main content
SpringerLink
Log in

Evaluation of Merremia tridentata (L.) Hallier f. for in vitro antioxidant activity

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Merremia tridentata (L.) Hallier f. is a member of Convolvulaceae family. This plant is used in the treatment of various ailments in folk medicine. In the present study, total phenolic contents and antioxidative properties of aerial parts and roots of M. tridentata were examined in different solvent extracts. The antioxidant activities were evaluated by measuring the ability of the extracts to scavenge the DPPH•, ABTS•+, and OH•. In addition, the reducing power, phosphomolybdenum reduction, Fe2+ chelation, antihemolytic activity, and inhibition of peroxidation were also assessed. The acetone extract of roots contained relatively higher levels of total phenolics (35.1 g/100 g extract), and possessed significant free radical scavenging and antioxidant properties. Whereas, the hot water extract of aerial parts exhibited the maximum iron chelation. The results obtained in the in vitro models clearly suggest that, M. tridentate is a natural source for antioxidants and validate the folkloric use of the plant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tapiero H, Tew KD, Nguyen BG, Mathé G. Polyphenols: Do they play a role in the prevention of human pathologies? Biomed. Pharmacother. 56: 200–207 (2002)

    Article  CAS  Google Scholar 

  2. Mitscher LA, Telikepalli H, McGhee E, Shankel DM. Natural antimutagenic agents. Mutat. Res. 350: 142–143 (1996)

    Google Scholar 

  3. Owen RW, Giacosa A, Hull WE, Haubner R, Spiegelhalder B, Bartsch H. Phenolic compounds isolated from olive oil. Eur. J. Cancer 36: 1235–1247 (2000)

    Article  CAS  Google Scholar 

  4. Sala A, Recio MD, Giner RM, Manez S, Tournier H, Schinella G, Rios JL. Anti-inflammatory and antioxidant properties of Helichrysum italicum. J. Pharm. Pharmacol. 54: 365–371 (2002)

    Article  CAS  Google Scholar 

  5. Pullaiah T. Encyclopaedia of World Medicinal Plants. Regency Publications, New Delhi, India. pp. 1338–1340 (2006)

    Google Scholar 

  6. Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Springer, New Delhi, India. pp. 410–411 (2007)

    Google Scholar 

  7. Hatapakki BC, Hukkeri V, Patil DN, Chavan MJ. Wound healing activity of Merremia tridentata. Indian Drugs 41: 532 (2004)

    Google Scholar 

  8. Kamalutheen M, Gopalakrishnan S, Ismail TS. Anti-inflammatory and anti-arthritic activities of Merremia tridentata (L.) Hall. f. E-J. Chem. 6: 943–948 (2009)

    CAS  Google Scholar 

  9. Aschfalk A, Steingass H, Muller W, Drochner E. Merremia tridentata as a supplementary feed to the grass Panicum maximum for young West African Dwarf sheep. Trop. Anim. Health Pro. 34: 45–50 (2002)

    Google Scholar 

  10. Siddhuraju P, Becker K. Studies on antioxidant activities of Mucuna seed (Mucuna pruriens var. utilis) extracts and certain non-protein amino/imino acids through in vitro models. J. Sci. Food Agr. 83: 1517–1524 (2003)

    Article  CAS  Google Scholar 

  11. Pulido R, Bravo L, Sauro-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J. Agr. Food Chem. 48: 3396–3402 (2000)

    Article  CAS  Google Scholar 

  12. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231–1237 (1999)

    Article  CAS  Google Scholar 

  13. Dinis TCP, Madeira VMC, Almeida LM. Action of phenolic derivatives (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophys. 315: 161–169 (1994)

    Article  CAS  Google Scholar 

  14. Prieto P, Pineda M, Aguilar M. Spectophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal. Biochem. 269: 337–341 (1999)

    Article  CAS  Google Scholar 

  15. Klein SM, Cohen G, Cederbaum AI. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry 20: 6006–6012 (1991)

    Article  Google Scholar 

  16. Blios MS. Antioxidants determination by the use of a stable free radical. Nature 26: 1199–1200 (1958)

    Article  Google Scholar 

  17. Taga MS, Miller EE, Pratt DE. Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 61: 928–931 (1984)

    Article  CAS  Google Scholar 

  18. Naim M, Gestener B, Bondi A, Birk Y. Antioxidant and antihemolytic activities of soyabean isoflavones. J. Agr. Food Chem. 24: 1174–1177 (1976)

    Article  CAS  Google Scholar 

  19. Govindarajan R, Singh DP, Rawat AKS. High-performance liquid chromatographic method for the quantification of phenolics in ‘Chyavanprash’ a potent Ayurvedic drug. J. Pharm. Biomed. Anal. 43: 527–532 (2007)

    Article  CAS  Google Scholar 

  20. Oktay M, Gulcin I, Kufrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. Lebensm. -Wiss. Technol. 36: 263–271 (2003)

    Article  CAS  Google Scholar 

  21. Huang DJ, Chen HJ, Wen-chi Houc W, Lin CD, Lin YH. Sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) storage root mucilage with antioxidant activities in vitro. Food Chem. 98: 774–781 (2006)

    Article  CAS  Google Scholar 

  22. Surveswaran S, Cai Y, Corke H, Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem. 102: 938–953 (2007)

    Article  CAS  Google Scholar 

  23. Halliwell B. Antioxidants; the basics-what they are and how to evaluate them. Adv. Pharmacol. 38: 3–20 (1997)

    Article  CAS  Google Scholar 

  24. Gulcin I, Elmastas M, Aboul-Enein HY. Determination of antioxidant and radical scavenging activity of basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytother. Res. 21: 354–361 (2007)

    Article  CAS  Google Scholar 

  25. Hyder RC, Lio ZD, Khodr HH. Metal chelation of polyphenols. Methods Enzymol. 335: 192–203 (2001)

    Google Scholar 

  26. Loo AY, Jain K, Darah I. Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chem. 107: 1151–1160 (2008)

    Article  CAS  Google Scholar 

  27. Wang H, Gao XD, Zhou GC, Cai L, Yao WB. In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chem. 106: 888–895 (2008)

    Article  CAS  Google Scholar 

  28. Jayaprakasha GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem. 73: 285–290 (2001)

    Article  CAS  Google Scholar 

  29. Djeridane A, Yousfi M, Nadjemi B, Vidal N, Lesgards JF, Stocker P. Screening of some Algerian medicinal plants for the phenolic compounds and their antioxidant activity. Eur. Food Res. Technol. 224: 801–809 (2006)

    Article  Google Scholar 

  30. Manian R, Anusuya N, Siddhuraju P, Manian, S. The antioxidant and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem. 107: 1000–1007 (2008)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    Kandhasamy Sowndhararajan, Jince Mary Joseph, Karuppusamy Arunachalam & Sellamuthu Manian

Authors
  1. Kandhasamy Sowndhararajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jince Mary Joseph
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Karuppusamy Arunachalam
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sellamuthu Manian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sellamuthu Manian.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sowndhararajan, K., Joseph, J.M., Arunachalam, K. et al. Evaluation of Merremia tridentata (L.) Hallier f. for in vitro antioxidant activity. Food Sci Biotechnol 19, 663–669 (2010). https://doi.org/10.1007/s10068-010-0093-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-010-0093-z

Keywords

Search

Navigation