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PGC-1α is associated with C2C12 Myoblast differentiation

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Central European Journal of Biology

Abstract

PGC-1α has been implicated as an important mediator of functional capacity of skeletal muscle. However, the role of PGC-1α in myoblast differentiation remains unexplored. In the present study, we observed a significant up-regulation of PGC-1α expression during the differentiation of murine C2C12 myoblast. To understand the biological significance of PGC-1α up-regulation in myoblast differentiation, C2C12 cells were transfected with murine PGC-1α cDNA and siRNA targeting PGC-1α, respectively. PGC-1α over-expressing clones fused to form typical myotubes with higher mRNA level of myosin heavy chain isoform I (MyHCI) and lower MyHCIIX. No obvious differentiation was observed in PGC-1α-targeted siRNA-transfected cells with marked decrement of mRNA levels of MyHCI and MyHCIIX. Furthermore, PGC-1α increased the expression of MyoD and MyoG in C2C12 cells, which controlled the commitment of precursor cells to myotubes. These results indicate that PGC-1α is associated with myoblast differentiation and elevates MyoD and MyoG expression levels in C2C12 cells.

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Authors and Affiliations

  1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China

    Yaqiu Lin, Yanying Zhao, Jiaqi Gong, Yucai Zheng & Yong Wang

  2. Reproductive and endocrine laboratory, Chengdu Woman-Child Central Hospital, Chengdu, 610051, China

    Ruiwen Li

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  1. Yaqiu Lin
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  2. Yanying Zhao
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  3. Ruiwen Li
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  4. Jiaqi Gong
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  5. Yucai Zheng
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  6. Yong Wang
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Correspondence to Yaqiu Lin.

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These authors contributed equally to this paper

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Lin, Y., Zhao, Y., Li, R. et al. PGC-1α is associated with C2C12 Myoblast differentiation. cent.eur.j.biol. 9, 1030–1036 (2014). https://doi.org/10.2478/s11535-014-0341-y

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  • DOI: https://doi.org/10.2478/s11535-014-0341-y

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