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Comparative evaluation of re-epithelialization promoted by fresh or cryopreserved cultured dermal substitute

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Abstract

Allogeneic cultured dermal substitute (CDS) was prepared by plating cultured fibroblasts on a two-layered spongy matrix of hyaluronic acid and atelocollagen, followed by culturing for 1 week. The resulting fresh CDS was then cryopreserved in a freezer at −152°C in accordance with conventional procedures. Fresh CDS was rinsed thoroughly with lactated Ringer’s solution to remove fetal bovine serum (FBS) and was then used in the clinical study, whereas cryopreserved CDS was thawed and then rinsed with lactated Ringer’s solution to remove cryoprotectant and FBS. The present study was designed to compare the efficacy of fresh and cryopreserved CDS in promoting reepithelialization at donor sites leaving behind split-thickness skin autografts. Fourteen donor sites were used for this comparative study. There were no differences in the period of complete re-epithelialization between the fresh and cryopreserved CDS. The results of this comparative study thus suggest that cryopreserved CDS is able to maintain the same level of potency to promote re-epithelialization as fresh CDS. This indicates that, although the release of growth factors is suppressed to some extent during the course of cryopreserving, thawing, and rinsing procedures, it is still sufficient to promote re-epithelialization.

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References

  1. Yamada N, Shioya N, Kuroyanagi Y. Evaluation of an allogeneic cultured dermal substitute composed of fibroblasts within a spongy collagen matrix as a wound dressing. Scand J Plast Reconstr Surg 1995;29:211–219

    Article  CAS  Google Scholar 

  2. Tanaka M, Nakakita N, Kuroyanagi Y. Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal tests. J Biomater Sci Polymer Edn 1999;10:433–453

    Article  CAS  Google Scholar 

  3. Yamada N, Uchinuma E, Kuroyanagi Y. Clinical evaluation of an allogeneic cultured dermal substitute composed of fibroblasts within a spongy collagen matrix. Scand J Plast Reconstr Surg 1999;33:147–154

    Article  CAS  Google Scholar 

  4. Yamada N, Uchinuma E, Kuroyanagi Y. Comparative evaluation of allogeneic cultured dermal substitute and allogeneic cultured epidermal sheet in promoting wound healing. J Jpn PRS 1999;19:610–616

    Google Scholar 

  5. Kuroyanagi Y, Yamada N, Yamashita R, Uchinuma E. Tissue-engineered product: allogeneic cultured dermal substitute composed of spongy collagen with fibroblasts. Artif Organs 2001;25:180–186

    Article  PubMed  CAS  Google Scholar 

  6. Kubo K, Kuroyanagi Y. Spongy matrix of hyaluronic acid and collagen as a cultured dermal substitute: evaluation in an animal test. J Artif Organs 2003;6:64–70

    Article  PubMed  CAS  Google Scholar 

  7. Kubo K, Kuroyanagi Y. Characterization of cultured dermal substitute composed of spongy matrix of hyaluronic acid and collagen combined with fibroblasts. J Artif Organs 2003;6:138–144

    PubMed  CAS  Google Scholar 

  8. Kubo K, Kuroyanagi Y. Development of a cultured dermal substitute composed of a spongy matrix of hyaluronic acid and atelocollagen combined with fibroblasts: fundamental evaluation. J Biomater Sci Polymer Edn 2003;14:625–641

    Article  CAS  Google Scholar 

  9. Kashiwa N, Ito O, Ueda T, Kubo K, Matsui H, Kuroyanagi Y. Treatment of full-thickness skin defect with concomitant grafting of sixfold extended mesh auto-skin and allogeneic cultured dermal substitute. Artif Organs 2004;28:444–450

    Article  PubMed  Google Scholar 

  10. Ohtani T, Okamoto K, Kaminaka C, Kishi T, Sakurane M, Yamamoto Y, Ueda K, Kubo K, Kuroyanagi Y, Furukawa F. Digital gangrene associated with idiopathic hypereosinophilia: treatment with allogeneic cultured dermal substitute (CDS). Eur J Dermatol 2004;14:168–171

    PubMed  Google Scholar 

  11. Moroi Y, Fujita S, Fukagawa S, Mashino T, Goto T, Masuda T, Urabe K, Kubo K, Matsui H, Kagawa S, Kuroyanagi Y, Furue M. Clinical evaluation of allogeneic cultured dermal substitutes for intractable skin ulcers after tumor resection. Eur J Dermatol 2004;14:172–176

    PubMed  Google Scholar 

  12. Hasegawa T, Suga Y, Mizoguchi M, Ikeda S, Ogawa H, Kubo K, Matsui H, Kagawa S, Kuroyanagi Y. Clinical trial of allogeneic cultured dermal substitute for the treatment of intractable skin ulcers in 3 patients with recessive dystrophic epidermolysis bullosa. J Am Acad Dermatol 2004;50:803–804

    Article  PubMed  Google Scholar 

  13. Hasegawa T, Suga Y, Mizoguchi M, Muramatsu S, Mizuno Y, Ogawa H, Kubo K, Kuroyanagi Y. An allogeneic cultured dermal substitute suitable for treating intractable skin ulcers and large skin defects prior to autologous skin grafting: three case reports. J Dermatol 2005;32:715–720

    PubMed  Google Scholar 

  14. Hasegawa T, Suga Y, Mizoguchi M, Muramatsu S, Mizuno Y, Haruna K, Ikeda S, Kuroyanagi Y, Ogawa H. Intractable venous leg ulcer treated successfully with allogeneic cultured dermal substitute. Scand J Plast Reconstr Surg Hand Surg 2007;41:326–328

    Article  PubMed  Google Scholar 

  15. Yonezawa M, Tanizaki H, Inoguchi N, Ishida M, Katoh M, Tachibana T, Miyachi Y, Kubo K, Kuroyanagi Y. Clinical study with allogeneic cultured dermal substitutes for chronic leg ulcers. Int J Dermatol 2007;46:36–42

    Article  PubMed  Google Scholar 

  16. Yamada N, Uchinuma E, Kuroyanagi Y. Clinical trial of allogeneic cultured dermal substitutes for intractable skin ulcers of the lower leg. J Artif Organs 2008;11:100–103

    Article  PubMed  CAS  Google Scholar 

  17. Kubo K, Kuroyanagi Y. Development of cultured dermal substitute composed of spongy matrix of hyaluronic acid and atelocollagen combined with fibroblasts: cryopreservation. Artif Organs 2004;28:182–188

    Article  PubMed  Google Scholar 

  18. Kubo K, Kuroyanagi Y. The possibility of long-term cryopreservation of cultured dermal substitute. Artif Organs 2005;29:800–805

    Article  PubMed  CAS  Google Scholar 

  19. Kuroyanagi Y, Kubo K, Matsui H, Kim HJ, Numari S, Mabuchi Y, Kagawa S. Establishment of banking system for allogeneic cultured dermal substitute. Artif Organs 2004;28:13–21

    Article  PubMed  Google Scholar 

  20. Kubo K, Kuroyanagi Y. Effects of vascular endothelial growth factor released from cultured dermal substitute on proliferation of vascular endothelial cells in vitro. J Artif Organs 2003;6:267–272

    Article  PubMed  CAS  Google Scholar 

  21. Kubo K, Kuroyanagi Y. A study of cytokines released from fibroblasts in cultured dermal substitute. Artif Organs 2005;29:845–849

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Plastic and Aesthetic Surgery, School of Medicine, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Japan

    Naoto Yamada & Eiju Uchinuma

  2. R & D Center for Artificial Skin, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan

    Yasuhiro Matsumoto & Yoshimitsu Kuroyanagi

Authors
  1. Naoto Yamada
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  2. Eiju Uchinuma
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  3. Yasuhiro Matsumoto
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  4. Yoshimitsu Kuroyanagi
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Correspondence to Naoto Yamada.

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Yamada, N., Uchinuma, E., Matsumoto, Y. et al. Comparative evaluation of re-epithelialization promoted by fresh or cryopreserved cultured dermal substitute. J Artif Organs 11, 221–224 (2008). https://doi.org/10.1007/s10047-008-0428-1

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  • DOI: https://doi.org/10.1007/s10047-008-0428-1

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