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Sirolimus-coated, poly(l-lactic acid)-modified polypropylene mesh with minimal intra-peritoneal adhesion formation in a rat model

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Abstract

Purpose

This study evaluated the manufacturing method and anti-adhesion properties of a new composite mesh in the rat model, which was made from sirolimus (SRL) grafts on a poly(l-lactic acid) (PLLA)-modified polypropylene (PP) hernia mesh.

Methods

PLLA was first grafted onto argon-plasma-treated native PP mesh through catalysis of stannous chloride. SRL was grafted onto the surface of PP-PLLA meshes using catalysis of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 4-dimethylaminopyridine (DMAP) in a CH2Cl2 solvent. Sprague–Dawley female rats received either SRL-coated meshes, PP-PLLA meshes, or native PP meshes to repair abdominal wall defects. At different intervals, rats were euthanized by a lethal dose of chloral hydrate and adhesion area and tenacity were evaluated. Sections of the mesh with adjacent tissues were assessed histologically.

Results

Attenuated total reflection Fourier transformed infrared (ATR-FTIR) spectroscopy indicated the existence of a C=O group absorption peak (1724.1 cm−1), and scanning electron microscope morphological analysis indicated that the surface of the PP mesh was covered with SRL. Compared to the native PP meshes and PP-PLLA meshes, SRL-coated meshes demonstrated the greatest ability to decrease the formation of adhesions (P < 0.05) and inflammation.

Conclusions

The SRL-coated composite mesh showed minimal formation of intra-abdominal adhesions in a rat model of abdominal wall defect repair.

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Acknowledgements

This work was financially supported by the Fundamental Research Funds for the Central Universities (2242016k40145) and National Natural Science Foundation of China (81600407).

Funding

This work was financially supported by the Fundamental Research Funds for the Central Universities (2242016k40145) and National Natural Science Foundation of China (81600407).

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Authors and Affiliations

  1. Department of General Surgery, Affiliated ZhongDa Hospital (Jiang Bei), Southeast University, No. 211 Jianmin Road, Nanjing, 210009, Jiangsu, China

    S. Lu

  2. Jiangsu Key Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, No. 2 Sipailou, Nanjing, 21009, China

    W. Hu & T. Zhang

  3. Department of General Surgery, Institute for Minimally Invasive Surgery, Affiliated ZhongDa Hospital, Medical School, Southeast University, No. 87 Dingjiaqiao Road, Nanjing, 210009, Jiangsu, China

    S. Lu, Z. Zhang & Z. Ji

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  1. S. Lu
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Corresponding author

Correspondence to Z. Ji.

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Conflict of interest

Shenglin Lu, Wanjun Hu, Zhigang Zhang, Zhenling Ji, and Tianzhu Zhang declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed.

Human and animal rights

All animal experiments were approved by the Institutional Animal Care and Use Committee of Southeast University and were in compliance with all regulatory guidelines.

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Lu, S., Hu, W., Zhang, Z. et al. Sirolimus-coated, poly(l-lactic acid)-modified polypropylene mesh with minimal intra-peritoneal adhesion formation in a rat model. Hernia 22, 1051–1060 (2018). https://doi.org/10.1007/s10029-018-1782-4

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  • DOI: https://doi.org/10.1007/s10029-018-1782-4

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