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Flow-through amperometric methods for detection of the bioactive compound quercetin: performance of glassy carbon and screen-printed carbon electrodes

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Abstract

Rapid methods using batch injection analysis (BIA) with amperometric detection were developed for the determination of quercetin extracted from the Brazilian plants Brunfelsia uniflora (BU), Vismia guianensis (VG), and Arrabidaea brachypoda (AB). One method was based on a glassy carbon electrode (GCE) and pulsed amperometry (BIA-PA), while the other employed a screen-printed carbon electrode (SPCE) and conventional amperometry (BIA-CA). Both proposed methods required minimal sample manipulation (only dilution in the carrier electrolyte), and the determination was performed with a single injection of 100 μL of sample solution. The BIA-PA method was highly accurate (RSD = 0.7%, n = 30), fast (120 injections h−1), and presented low limits of detection (0.004 μmol L−1) and quantification (0.015 μmol L−1). The BIA-CA method was also accurate (RSD = 1.1%, n = 30), fast (120 injections h−1), and presented low limits of detection (0.027 μmol L−1) and quantification (0.091 μmol L−1). The application of the proposed methods for the determination of quercetin in the plant extracts provided results similar to those obtained by high-performance liquid chromatography (HPLC) at a confidence level of 95%.

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Funding

The authors are grateful for the financial support provided by the Brazilian agencies: Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA) (grant numbers #Universal-01372/17 (LMFD), #Universal-00827/17 (AAT)), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grant numbers 465389/2014-7 (AAT), #133321/2019-3 (WBV), #205220/2018-5 (ISS)), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Finance Code 001).

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Authors and Affiliations

  1. Departamento de Química, Universidade Federal do Maranhão, Av dos Portugueses 1966 - CEP 65.080-805, São Luís, MA, Brazil

    Geyse Adriana Corrêa Ribeiro, Cláudia Quintino da Rocha & Auro Atsushi Tanaka

  2. Departamento de Tecnologia Química, Universidade Federal do Maranhão, Av dos Portugueses 1966 - CEP 65.080-805, São Luís, MA, Brazil

    William Barros Veloso, Luiza Maria Ferreira Dantas & Iranaldo Santos da Silva

  3. Instituto de Química, Universidade Federal de Uberlândia, Av. João Naves de Ávila 2121, CEP 38400-902, Uberlândia, MG, Brazil

    Eduardo Mathias Richter

  4. Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Caixa Postal 6154, CEP 13083-970, Campinas, SP, Brazil

    Eduardo Mathias Richter & Auro Atsushi Tanaka

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  1. Geyse Adriana Corrêa Ribeiro
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  2. Cláudia Quintino da Rocha
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  3. William Barros Veloso
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  4. Luiza Maria Ferreira Dantas
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Ribeiro, G.A.C., da Rocha, C.Q., Veloso, W.B. et al. Flow-through amperometric methods for detection of the bioactive compound quercetin: performance of glassy carbon and screen-printed carbon electrodes. J Solid State Electrochem 24, 1759–1768 (2020). https://doi.org/10.1007/s10008-020-04599-x

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