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Simultaneous determination of guanine, adenine, thymine and cytosine with a simple electrochemical method

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Abstract

A simple method for simultaneous detection of guanine, adenine, thymine and cytosine was set up by using a bare glassy carbon electrode in acetate buffer solution of pH 4.5. The peak current responses of these four DNA bases in this supporting electrolyte were significantly increased comparing with those in phosphate buffer solution and Tris-HCl, moreover, the peak current values were linearly dependent on the concentration of four DNA bases, respectively. Individual and simultaneous determinations of four bases were performed by controlling certain experimental conditions, and broad linear ranges and low detection limits (S/N = 3) were obtained. The assay processes do not need any separation or pretreatment steps. In addition, this method showed good selectivity, reproducibility, and stability and can be used for determination of the four bases content in real DNA sample.

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Acknowledgments

We are pleased to acknowledge the financial support of the China Postdoctoral Science Foundation (No. 2015 M572039), Natural Science Foundation of Shandong Province, China (No. BS2013HZ027), the National Natural Science Foundation of China (No. 21105023), and the Shandong Province High School Science and Technology Planning Project (No. J13LD51).

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Authors and Affiliations

  1. School of Chemical Engineering, China University of Mining and Technology, Xuzhou, 221116, Jiangsu, People’s Republic of China

    Hongying Li & Wei Zhao

  2. Department of Chemistry and Chemical Engineering, Heze University, Heze, 274015, Shandong, People’s Republic of China

    Hongying Li, Xueliang Wang & Zhaoxia Wang

Authors
  1. Hongying Li
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  2. Xueliang Wang
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  3. Zhaoxia Wang
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  4. Wei Zhao
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Corresponding author

Correspondence to Wei Zhao.

Electronic supplementary materials

ESM 1

This section consists of Fig S1, Table S1 and Table S2. Fig. S1 shows the DPVs of 50 μM G, 50 μM A, 100 μM T and 100 μM C in various pH ABS. Table S1 shows the signal change of various foreign species on the peak currents of 20 μM G, 20 μM A, 50 μM T and 50 μM C in ABS (pH 4.5). Table S2 exhibits the determination of G, A, T and C using standard addition method in real samples (n = 3). (DOCX 64.8 kb)

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Li, H., Wang, X., Wang, Z. et al. Simultaneous determination of guanine, adenine, thymine and cytosine with a simple electrochemical method. J Solid State Electrochem 20, 2223–2230 (2016). https://doi.org/10.1007/s10008-016-3227-2

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  • DOI: https://doi.org/10.1007/s10008-016-3227-2

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