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Indicator-free electrochemical genosensing originated from the self-signal of poly-xanthurenic acid enhanced by Fe3O4/reduced graphene oxide

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Abstract

In this paper, an indicator-free electrochemical genosensing platform based on the self-signal changes of poly-xanthurenic acid (PXa) enhanced by Fe3O4/reduced graphene oxide (Fe3O4/RGO) was constructed. The resulting nanocomposite (PXa-Fe3O4/RGO) was characterized by transmission electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The π–π* stacking and hydrogen bonding between the conjugated Fe3O4/GO and aromatic ring of xanthurenic acid monomer promoted the electropolymerization efficiency accompanied with an increased electrochemical response of PXa. The immobilization of the specific probe DNA was successfully realized via the noncovalent method due to the π–π* interaction between the conjugated nanostructure of PXa-Fe3O4/RGO and DNA bases. The hybridization between the probe DNA and target DNA induced the resulted double-stranded (ds)DNA to be released from the conjugated nanocomposite, accompanied with the self-signal regeneration of nanocomposite (“signal-on”). The self-signal changes could serve as a powerful tool for indicator-free and freely switchable detection of different target genes, and the synergistic effect of the integrated graphene-based nanocomposite effectively improved the sensitivity for the target DNA detection via EIS.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 21205057, 21101087, 21375057), Shandong Province Natural Science Foundation (No. ZR2010BQ030), and the Doctoral Fund and Scientific Research Project of Linyi University.

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Authors and Affiliations

  1. Key Laboratory of Resources and Environmental Analytical Chemistry in Universities of Shandong, School of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, China

    Wei Zhang, Libin Wang & Xiuwen Zheng

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  1. Wei Zhang
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  2. Libin Wang
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  3. Xiuwen Zheng
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Correspondence to Wei Zhang or Xiuwen Zheng.

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Zhang, W., Wang, L. & Zheng, X. Indicator-free electrochemical genosensing originated from the self-signal of poly-xanthurenic acid enhanced by Fe3O4/reduced graphene oxide. J Solid State Electrochem 18, 2367–2373 (2014). https://doi.org/10.1007/s10008-014-2487-y

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  • DOI: https://doi.org/10.1007/s10008-014-2487-y

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