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Development of DNA monitoring platform based on poly(xanthurenic acid) functionalized FePt/reduced graphene oxide

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Abstract

A freely switchable DNA monitoring platform based on poly(xanthurenic acid) functionalized FePt/reduced graphene oxide (PXa-FePt/RGO) was developed, where the PXa and FePt/RGO were synchronously synthesized via an effective pulse potentiostatic method. The obtained nanocomposite integrated the advantages of the PXa and FePt/RGO, including rich-conjugated structures, good conductivity, and fine biocompatibility, which not only served as a substrate for DNA immobilization but also reflected the electrochemical transduction originating from DNA immobilization and hybridization without any complicated labeling or outer indicators. The immobilization of probe DNA was successfully conducted via noncovalent assembly due to the π–π* interaction between the conjugated nanocomposite and DNA bases. After the hybridization of probe DNA with target DNA, the formation of double-helix structure induced dsDNA to release from the surface of conjugated nanocomposite, accompanied with the self-signal regeneration of nanocomposite (“signal-on”). The developed biosensor exhibited excellent performance for the detection of the sequence-specific DNA from chronic myelogenous leukemia by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with a detection limit of 2.1 × 10−16 mol/L.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 21205057, 21101087, and 21375057), National Undergraduate Training Program for Innovation and Entrepreneurship (No. 201410452008), Undergraduate Training Program for Innovation and Entrepreneurship of Linyi University (No. 201410452008), and the Doctoral Fund and Scientific Research Project of Linyi University.

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  1. School of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, China

    Wei Zhang & Yanchao Su

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  1. Wei Zhang
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  2. Yanchao Su
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Correspondence to Wei Zhang.

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Zhang, W., Su, Y. Development of DNA monitoring platform based on poly(xanthurenic acid) functionalized FePt/reduced graphene oxide. J Solid State Electrochem 19, 1285–1291 (2015). https://doi.org/10.1007/s10008-015-2748-4

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  • DOI: https://doi.org/10.1007/s10008-015-2748-4

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