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Morphological study of efficacy of clarithromycin-loaded nanocarriers for treatment of biofilm infection disease

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Abstract

In this study, we developed a drug delivery system (DDS) using polymeric nanocarriers for the treatment of biofilm infection disease. Clarithromycin (CAM)-encapsulated and chitosan (CS) modified polymeric nanoparticles (NPs) were prepared using a polyvinyl caprolactam—polyvinyl acetate—polyethylene glycol graft copolymer (Soluplus®) (Sol) and poly-(DL-lactide-co-glycolide), respectively. To understand the availability of the prepared NPs, we made morphological observations of the antibacterial activity derived from the NPs toward the bacterial cells within the biofilm using scanning electron microscopy and transmission electron microscopy measurements. These results revealed different antibacterial activities for the two types of drug carriers. In the case of CAM-encapsulated + CS-modified Sol micelles treatment, NPs can exert their antibacterial activity not only by the surfactant, CAM and CS effects but also by intrusion into the bacterial cells. Thereby, CAM-encapsulated + CS-modified Sol micelles had a higher antibacterial activity. The morphological information is useful to design suitable NPs for the treatment against biofilm infections.

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Acknowledgments

This study was partially supported by JSPS KAKENHI Grant Numbers 25460046, 50574448 and NIMS microstructural characterization platform as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. The authors are grateful to Prof. S. Yamada, of Kawasaki Medical University, Japan and Prof. Y. Nishiyama, of Teikyo University, Japan for useful discussions regarding TEM observation.

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Authors and Affiliations

  1. School of Pharmacy, Pharmaceutical Engineering, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan

    Chisato Takahashi, Yuki Akachi, Noriko Ogawa, Yoshiaki Kawashima & Hiromitsu Yamamoto

  2. Department of Oral Anatomy, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi, 464-8650, Japan

    Keiichi Moriguchi

  3. Department of Materials Science and Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi, 466-8555, Japan

    Toru Asaka

  4. Department of Frontier Materials, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi, 466-8555, Japan

    Masaki Tanemura

Authors
  1. Chisato Takahashi
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  2. Yuki Akachi
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  3. Noriko Ogawa
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  4. Keiichi Moriguchi
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  5. Toru Asaka
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  6. Masaki Tanemura
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  7. Yoshiaki Kawashima
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  8. Hiromitsu Yamamoto
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Correspondence to Chisato Takahashi.

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Takahashi, C., Akachi, Y., Ogawa, N. et al. Morphological study of efficacy of clarithromycin-loaded nanocarriers for treatment of biofilm infection disease. Med Mol Morphol 50, 9–16 (2017). https://doi.org/10.1007/s00795-016-0141-8

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  • DOI: https://doi.org/10.1007/s00795-016-0141-8

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