Abstract
We applied the Hilbert differential contrast phase electron microscopy technique for the first time to mammalian cells, Ptk2 cells. Intracellular architectures such as the cytoskeletal network, membranous organelles, and mitochondria were observed without prior removal of cell membranes or extraction of soluble proteins. The attachment of mitochondria and membrane organelles with microtubules were observed. Microtubules were depolymerized by nocodazole treatment as expected. Thus, Hilbert differential contrast phase electron microscopy of vitrified cells is a nano-scale molecular imaging technique that opens up new vistas for exploring the supramolecular organization of the mammalian cell.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
O Medalia I Weber AS Frangakis D Nicastro G Gerisch W Baumeister (2002) ArticleTitleMacromolecular architecture in eukaryotic cells visualized by cryoelectron tomography Science 298 1209–1213 Occurrence Handle12424373 Occurrence Handle10.1126/science.1076184 Occurrence Handle1:CAS:528:DC%2BD38XosVertbY%3D
F Zernike (1955) ArticleTitleHow I discovered phase contrast Science 121 345–349 Occurrence Handle13237991 Occurrence Handle10.1126/science.121.3141.345 Occurrence Handle1:STN:280:CyqD2cbosF0%3D
JC Cheetham PJ Artymiuk DC Phillips (1992) ArticleTitleRefinement of an enzyme complex with inhibitor bound at partial occupancy. Hen egg-white lysozyme and tri-N-acetylchitotriose at 1.75 A resolution J Mol Biol 224 613–628 Occurrence Handle1569548 Occurrence Handle10.1016/0022-2836(92)90548-X Occurrence Handle1:CAS:528:DyaK38XitlSksb0%3D
R Danev K Nagayama (2001) ArticleTitleTransmission electron microscopy with Zernike phase plate Ultramicroscopy 88 243–252 Occurrence Handle11545320 Occurrence Handle10.1016/S0304-3991(01)00088-2 Occurrence Handle1:CAS:528:DC%2BD3MXlslChuro%3D
H Boersch (1947) ArticleTitleÜber die Kontraste von Atomen in Electronenmikroskop Z Naturforsch AA Phys Sci 2 IssueID11–1 615–633
AJ Koster R Grimm D Typke R Hegerl A Stoschek J Walz W Baumeister (1997) ArticleTitlePerspectives of molecular and cellular electron tomography J Struct Biol 120 276–308 Occurrence Handle9441933 Occurrence Handle10.1006/jsbi.1997.3933 Occurrence Handle1:CAS:528:DyaK1cXosleiug%3D%3D
BF McEwen M Marko (2001) ArticleTitleThe emergence of electron tomography as an important tool for investigating cellular ultrastructure J Histochem Cytochem 49 553–564 Occurrence Handle11304793 Occurrence Handle1:CAS:528:DC%2BD3MXjs1ahsrc%3D
K Danov R Danov K Nagayama (2002) ArticleTitleReconstruction of the electric charge density in thin films from the contrast transfer function measurements Ultramicroscopy 90 85–95 Occurrence Handle10.1016/S0304-3991(01)00143-7 Occurrence Handle1:CAS:528:DC%2BD38XhslWjs7w%3D
MJ Grimm JL Williams (1997) ArticleTitleAssessment of bone quantity and ‘quality’ by ultrasound attenuation and velocity in the heel Clin Biomech (Bristol, Avon) 12 281–285 Occurrence Handle10.1016/S0268-0033(97)00014-4
D Nicastro AS Frangakis D Typke W Baumeister (2000) ArticleTitleCryo-electron tomography of neurospora mitochondria J Struct Biol 129 48–56 Occurrence Handle10675296 Occurrence Handle10.1006/jsbi.1999.4204 Occurrence Handle1:STN:280:DC%2BD3c7ktFSqtA%3D%3D
K Nagayama (2005) ArticleTitlePhase contrast enhancement with phase plates in electron microscopy Adv Imaging Electr Phys 135 69–146
A Muller-Taubenberger AN Lupas H Li M Ecke E Simmeth G Gerisch (2001) ArticleTitleCalreticulin and calnexin in the endoplasmic reticulum are important for phagocytosis EMBO J 20 6772–6782 Occurrence Handle11726513 Occurrence Handle10.1093/emboj/20.23.6772 Occurrence Handle1:CAS:528:DC%2BD3MXptVaqtbw%3D
M Setou DH Seog Y Tanaka Y Kanai Y Takei M Kawagishi N Hirokawa (2002) ArticleTitleGlutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites Nature (Lond) 417 83–87 Occurrence Handle10.1038/nature743 Occurrence Handle1:CAS:528:DC%2BD38XjsFOgur0%3D
M Setou T Nakagawa DH Seog N Hirokawa (2000) ArticleTitleKinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport Science 288 1796–1802 Occurrence Handle10846156 Occurrence Handle10.1126/science.288.5472.1796 Occurrence Handle1:CAS:528:DC%2BD3cXjvFCisrs%3D
M Setou T Hayasaka I Yao (2004) ArticleTitleAxonal transport versus dendritic transport J Neurobiol 58 201–206 Occurrence Handle14704952 Occurrence Handle10.1002/neu.10324
C Zhao J Takita Y Tanaka M Setou T Nakagawa S Takeda HW Yang S Terada T Nakata Y Takei M Saito S Tsuji Y Hayashi N Hirokawa (2001) ArticleTitleCharcot-Marie-Tooth disease type 2A caused by mutation in a microtubule motor KIF1Bbeta Cell 105 587–597 Occurrence Handle11389829 Occurrence Handle10.1016/S0092-8674(01)00363-4 Occurrence Handle1:CAS:528:DC%2BD3MXktlSqtrw%3D
Y Kaneko R Danev K Nitta K Nagayama (2005) ArticleTitleIn vivo subcellular ultrastructures recognized with Hilbert differential contrast transmission electron microscopy J Electron Microsc (Tokyo) 54 79–84 Occurrence Handle10.1093/jmicro/dfh105
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Setou, M., Radostin, D., Atsuzawa, K. et al. Mammalian cell nano structures visualized by cryo Hilbert differential contrast transmission electron microscopy. Med Mol Morphol 39, 176–180 (2006). https://doi.org/10.1007/s00795-006-0341-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00795-006-0341-8