Abstract
The level of plasma diamine oxidase (DAO) activity is associated with the maturation and integrity of small intestinal mucosa. This study in rats investigated whether a decreased level of plasma DAO could reflect the severity of mucosal injury due to intravenous 5-fluorouracil (5-FU) treatment. The beneficial effect of soluble dietary fiber (SDF) on preventing diarrhea after 5-FU treatment was also examined. To induce diarrhea, 5-FU (50 mg/kg/day for four days) was administered via the tail vein with or without SDF supplementation. After 5-FU treatment, the majority of rats developed moderate to severe diarrhea, and levels of plasma DAO activity significantly decreased compared to those of control group (P < 0.05). Scanning electron microscopy revealed disarrangement of the small intestinal villi. Contrarily, the rats supplemented with SDF had diarrhea less frequently (50.0 vs. 91.7 %, P = 0.025) on day five, and DAO activity levels were significantly higher than in those rats administered 5-FU alone (8.25 ± 5.34 vs. 5.50 ± 4.32, P = 0.023). In conclusion, plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment, and SDF supplementation might be a practical and useful treatment for reducing the intestinal toxicity of 5-FU.
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Acknowledgments
Part of this study was reported at a workshop during the 43th annual meeting of the Japanese Society for Clinical Molecular Morphology and in a symposium presented to the 44th annual meeting of the Japanese Society for Clinical Molecular Morphology. We are grateful to Mr. Kenichi Yagyuu and Ms. Yuka Takezaki for their excellent technical assistance. This work was supported by a Grant in Aid for Scientific Research (22501045).
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Fukudome, I., Kobayashi, M., Dabanaka, K. et al. Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats. Med Mol Morphol 47, 100–107 (2014). https://doi.org/10.1007/s00795-013-0055-7
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DOI: https://doi.org/10.1007/s00795-013-0055-7