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Developments and applications of mass microscopy

  • Award Review
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Abstract

We have developed a mass microscopy technique, i.e., a microscope combined with high-resolution matrixassisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS), which is a powerful tool for investigating the spatial distribution of biomolecules without any time-consuming extraction, purification, and separation procedures for biological tissue sections. Mass microscopy provides clear images about the distribution of hundreds of biomolecules in a single measurement and also helps in understanding the cellular profile of the biological system. The sample preparation and the spatial resolution and speed of the technique are all important steps that affect the identification of biomolecules in mass microscopy. In this Award Lecture Review, we focus on some of the recent developments in clinical applications to show how mass microscopy can be employed to assess medical molecular morphology.

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Author information

Authors and Affiliations

  1. Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan

    Mitsutoshi Setou, Kamlesh Shrivas, Morakot Sroyraya, Hyunjeong Yang, Yuki Sugiura, Junji Moribe, Akira Kondo, Koji Tsutsumi, Yoshishige Kimura, Nobuya Kurabe, Takahiro Hayasaka, Naoko Goto-Inoue, Nobuhiro Zaima, Koji Ikegami & Yoshiyuki Konishi

  2. Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand

    Morakot Sroyraya & Prasert Sobhon

Authors
  1. Mitsutoshi Setou
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  2. Kamlesh Shrivas
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  3. Morakot Sroyraya
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  4. Hyunjeong Yang
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  5. Yuki Sugiura
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  6. Junji Moribe
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  7. Akira Kondo
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  8. Koji Tsutsumi
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  9. Yoshishige Kimura
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  10. Nobuya Kurabe
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  11. Takahiro Hayasaka
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  12. Naoko Goto-Inoue
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  13. Nobuhiro Zaima
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  14. Koji Ikegami
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  15. Prasert Sobhon
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  16. Yoshiyuki Konishi
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Corresponding author

Correspondence to Mitsutoshi Setou.

Additional information

Dr. Mitsutoshi Setou, of the Department of Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, is the winner of the Japanese Society for Clinical Molecular Morphology Award for Promoting Young Researchers in 2009. Dr. Setou is recognized for his great contribution in the development of mass microscopy and its application in the field of medical molecular morphology.

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Setou, M., Shrivas, K., Sroyraya, M. et al. Developments and applications of mass microscopy. Med Mol Morphol 43, 1–5 (2010). https://doi.org/10.1007/s00795-009-0489-0

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  • DOI: https://doi.org/10.1007/s00795-009-0489-0

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