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Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans

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Abstract

Alkaliphilic Bacillus sp. AH-101 was characterized in terms of physiological and biochemical characteristics, and 16S rDNA sequence homology and DNA–DNA hybridization analyses were performed. Phylogenetic analysis of strain AH-101 based on comparison of 16S rDNA sequences revealed that this strain is closely related to Bacillus halodurans. DNA–DNA hybridization of AH-101 and related Bacillus reference strains showed that the highest level of DNA–DNA relatedness (88%) was found between strain AH-101 and the B. halodurans type strain (DSM497). Our findings demonstrate that strain AH-101 is a member of the species B. halodurans.

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Authors and Affiliations

  1. Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp, , , , , , JP

    Hideto Takami, Yuichi Nogi & Koki Horikoshi

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  1. Hideto Takami
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  2. Yuichi Nogi
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  3. Koki Horikoshi
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Received: June 10, 1999 / Accepted: August 6, 1999

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Takami, H., Nogi, Y. & Horikoshi, K. Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans . Extremophiles 3, 293–296 (1999). https://doi.org/10.1007/s007920050130

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  • DOI: https://doi.org/10.1007/s007920050130

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