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Filamentous anoxygenic phototrophic bacteria from cyanobacterial mats of Alla hot springs (Barguzin Valley, Russia)

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Abstract

Alkaline hydrotherms of the Baikal rift zone are unique systems to study the diversity of thermophilic bacteria. In this study, we present data on the phototrophic bacterial community of cyanobacterial mats from the alkaline Alla hot spring. Using a clonal analysis approach, this study evaluated the species diversity, the proportion of oxygenic and anoxygenic phototrophs and their distribution between various areas of the spring. Novel group-specific PCR primers were designed and applied to detect representatives of the Chloroflexus and Roseiflexus genera in mat samples. For the first time, the presence of Roseiflexus-like bacteria was detected in the Baikal rift zone.

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Acknowledgments

The authors are grateful to colleagues of the Laboratory of Microbiology at the Institute of General and Experimental Biology, Siberian Branch, RAS, Ulan-Ude, for organizing the expedition to the thermal springs of the Barguzin Valley. The authors are also grateful to Kolganova T.V. and Baslerov R.V., of the Molecular Diagnostics Laboratory, Centre for Bioengineering RAS. The kind assistance of B. Dolphin-Nightingale, from the University of Portsmouth, in manuscript preparation is gratefully acknowledged. The work was carried out using the scientific equipment of Core Research Facility of Centre for Bioengineering, RAS with support from the Russian Foundation for Basic Research, projects ## 15-04-07655 and 15-04-01275.

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Authors and Affiliations

  1. Centre Bioengineering RAS, Prospekt 60-Letiya Oktyabrya, 7/1, 117312, Moscow, Russia

    Vasil A. Gaisin, Marina V. Sukhacheva & Boris B. Kuznetsov

  2. Winogradsky Institute of Microbiology RAS, Prospekt 60-Letiya Oktyabrya, 7/2, 117312, Moscow, Russia

    Alexander M. Kalashnikov, Zorigto B. Namsaraev & Vladimir M. Gorlenko

  3. Institute of General and Experimental Biology RAS, Sakhyanovoy st., 6, 670047, Ulan-Ude, Russia

    Darima D. Barhutova

  4. National Research Centre “Kurchatov Institute”, Akademika Kurchatova pl., 1, 123182, Moscow, Russia

    Zorigto B. Namsaraev

Authors
  1. Vasil A. Gaisin
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  2. Alexander M. Kalashnikov
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  3. Marina V. Sukhacheva
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  4. Zorigto B. Namsaraev
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  5. Darima D. Barhutova
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  6. Vladimir M. Gorlenko
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  7. Boris B. Kuznetsov
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Correspondence to Boris B. Kuznetsov.

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Communicated by A. Oren.

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Gaisin, V.A., Kalashnikov, A.M., Sukhacheva, M.V. et al. Filamentous anoxygenic phototrophic bacteria from cyanobacterial mats of Alla hot springs (Barguzin Valley, Russia). Extremophiles 19, 1067–1076 (2015). https://doi.org/10.1007/s00792-015-0777-7

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  • DOI: https://doi.org/10.1007/s00792-015-0777-7

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