Abstract
A novel haloalkaliphilic sulfate-reducing bacterium, designated Al915-01T, was isolated from benthic sediments of the Lake Alginskoe, a soda lake located in the Trans-Baikal Region, Russia. Cells of the strain were Gram-stain negative, motile, non-spore-forming vibrion (0.4–0.5 × 1.2–2.3 µm). Strain Al915-01T grew in the pH range from 8.0 to 10.5 (optimum pH 9.0) and required NaCl for growth (5–100 g l−1 NaCl, optimum 40 g l−1). The bacterium grew at 10–40 °C (optimally at 36 °C) and used lactate, formate and pyruvate as electron donors in the presence of sulfate. It was able to reduce sulfate, sulfite, thiosulfate and nitrate with lactate as an electron donor. The isolate was able to grow lithoheterotrophically with sulfate and molecular hydrogen if acetate was added as a carbon source. The predominant fatty acids were anteisoC15:0, isoC17:1, C18:1ω7 and C16:1ω7. The G+C content in the DNA was 58.3 ± 1 mol %. Analysis of the 16S rRNA gene sequence showed that the new bacterium belongs to the genus Desulfonatronum. The closest relatives were Desulfonatronum buryatense Ki5T (99.9 % similarity) and Desulfonatronum lacustre Z-7951T (99.2 % similarity). On the basis of the genotypic, phenotypic and phylogenetic characteristics, the isolate is proposed as a representative of a novel species Desulfonatronum zhilinae with the type strain Al915-01T (=VKM B-2744T = DSM 26338T).
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Acknowledgments
We thank Dr N. A. Kostrikina (Winogradsky Institute of Microbiology, RAS) for assistance with electron microscopy, Dr Y. Osipov (Bakulev Centre of Cardiovascular Surgery, Moscow, Russia) for the cell-envelope fatty acid assay and Dr A. Avtukh (Skryabin Institute of Biochemistry and Physiology of Microorganisms RAS) for the organic acids assay. The work was supported by an RFBR grant (Project No. 12-04-31353) and the Ministry of Education and Science of the Russian Federation (Contract No. 14.518.11.7069).
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Zakharyuk, A.G., Kozyreva, L.P., Khijniak, T.V. et al. Desulfonatronum zhilinae sp. nov., a novel haloalkaliphilic sulfate-reducing bacterium from soda Lake Alginskoe, Trans-Baikal Region, Russia. Extremophiles 19, 673–680 (2015). https://doi.org/10.1007/s00792-015-0747-0
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DOI: https://doi.org/10.1007/s00792-015-0747-0