Abstract
Amongst more than 1000 isolates collected in various cold environments, the strain Arthrobacter psychrolactophilus Sp 31.3 has been selected for its ability to grow and to produce exoenzymes at low temperatures, its inability to grow at 37°C, its non-halophilic character and its growth versatility on various media. This non-pathogenic strain displays a strong resistance to desiccation and storage at room temperature and is suitable for the production of freeze-dried bacterial starters. When grown in a synthetic wastewater at 10°C, the strain induces a complete clarification of the turbid medium and efficiently hydrolyses proteins, starch and lipids in the broth. Furthermore, this strain has a remarkable capacity to improve the biodegradability of organic compounds in wastewater as indicated by a BOD5/COD ratio of 0.7.
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References
Aislabie J, Saul DJ, Foght JM (2006) Bioremediation of hydrocarbon-contaminated polar soils. Extremophiles 10:171–179
Bazot S, Bois P, Joyeux C, Lebeau T (2007) Mineralization of diuron [3-(3, 4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized Arthrobacter sp. and Delftia acidovorans. Biotechnol Lett 29:749–754
Bernfeld P (1955) Amylases, α and β. Methods Enzymol 1:149–151
Carlone GM, Valadez MJ, Pickett MJ (1982) Methods for distinguishing gram-positive from gram-negative bacteria. J Clin Microbiol 16:1157–1159
D’Amico S, Collins T, Marx JC, Feller G, Gerday C (2006) Psychrophilic microorganisms: challenges for life. EMBO Rep 7:385–389
Daims H, Taylor MW, Wagner M (2006) Wastewater treatment: a model system for microbial ecology. Trends Biotechnol 24:483–489
El Fantroussi S, Agathos SN (2005) Is bioaugmentation a feasible strategy for pollutant removal and site remediation? Curr Opin Microbiol 8:268–275
Feller G, Gerday C (2003) Psychrophilic enzymes: hot topics in cold adaptation. Nat Rev Microbiol 1:200–208
Jorgensen KS (2007) In situ bioremediation. Adv Appl Microbiol 61:285–305
Margesin R, Schinner F (1998) Low-temperature bioremediation of a waste water contaminated with anionic surfactants and fuel oil. Appl Microbiol Biotechnol 49:482–486
Margesin R, Schinner F (1999) Biotechnological applications of cold-adapted organisms. Springer, Heidelberg
Margesin R, Schinner F (2001) Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl Microbiol Biotechnol 56:650–663
Margesin R, Feller G, Gerday C, Russell NJ (2002) Cold-adapted microorganisms: adaptation strategies and biotechnological potential. In: Bitton G (ed) Encyclopedia of environmental microbiology, vol 2. Wiley, New York, pp 871–885
Mongodin EF, Shapir N, Daugherty SC, DeBoy RT, Emerson JB, Shvartzbeyn A, Radune D, Vamathevan J, Riggs F, Grinberg V, Khouri H, Wackett LP, Nelson KE, Sadowsky MJ (2006) Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1. PLoS Genet 2:e214
Schatz A, Bovell C Jr (1952) Growth and hydrogenase activity of a new bacterium, Hydrogenomonas facilis. J Bacteriol 63:87–98
Sharma NK, Pandey J, Gupta N, Jain RK (2007) Growth and physiological response of Arthrobacter protophormiae RKJ100 toward higher concentrations of o-nitrobenzoate and p-hydroxybenzoate. FEMS Microbiol Lett 271:65–70
Singer AC, van der Gast CJ, Thompson IP (2005) Perspectives and vision for strain selection in bioaugmentation. Trends Biotechnol 23:74–77
Spies JR (1957) Colorimetric procedures for amino acids. Methods Enzymol 3:467–477
van Veen JA, van Overbeek LS, van Elsas JD (1997) Fate and activity of microorganisms introduced into soil. Microbiol Mol Biol Rev 61:121–135
Vogel TM (1996) Bioaugmentation as a soil bioremediation approach. Curr Opin Biotechnol 7:311–316
Wang X, Liu X, Wang H, Dong Q (2007) Utilization and degradation of imazaquin by a naturally occurring isolate of Arthrobacter crystallopoietes. Chemosphere 67:2156–2162
Acknowledgments
This work was supported by the Région Wallonne, Belgium (First Europe contract Biorem) and by the Fonds de la Recherche Scientifique-FNRS, Belgium (FRFC grants). The facilities offered by the Institut Polaire Français are also acknowledged. We thank S. Shivaji (Hyderabad, India) for providing the CMS strains. SD’A was a FNRS post-doctoral researcher.
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Communicated by T. Matsunaga.
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Gratia, E., Weekers, F., Margesin, R. et al. Selection of a cold-adapted bacterium for bioremediation of wastewater at low temperatures. Extremophiles 13, 763–768 (2009). https://doi.org/10.1007/s00792-009-0264-0
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DOI: https://doi.org/10.1007/s00792-009-0264-0