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Effects of pressure on cell morphology and cell division of lactic acid bacteria

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Abstract

The effect of pressure and temperature on the growth of the mesophilic lactic acid bacteria Lactococcus lactis and Lactobacillus sanfranciscensis was studied. Both strains were piezosensitive. Lb. sanfranciscensis failed to grow at 50 MPa and the growth rate of Lc. lactis at 50 MPa was less than 30% of that at atmospheric pressure. An increase of growth temperature did not improve the piezotolerance of either organism. During growth under high-pressure conditions, the cell morphology was changed, and the cells were elongated as cell division was inhibited. At atmospheric pressure, temperatures above the optimal temperature for growth caused a similar effect on cell morphology and cell division in both bacteria as that observed under high-pressure conditions. The segregation and condensation of chromosomal DNA were observed by DAPI staining and occurred normally at high-pressure conditions independent of changes in cell morphology. Immunofluorescence microscopy of Lc. lactis cells demonstrated an inhibitory effect of high pressure on the formation of the FtsZ ring and this inhibition of the FtsZ ring formation is suggested to contribute to the altered cell morphology and growth inhibition induced by high pressure.

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Acknowledgments

This work was supported by grant No. FOR 358/1 of the Deutsche Forschungsgemeinschaft. We wish to thank the technicians of the DEEP-BATH system for their cooperation to this work.

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Authors and Affiliations

  1. Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350, Freising, Germany

    Adriana Molina-Höppner, Michael G. Gänzle & Rudi F. Vogel

  2. Department of Marine Ecosystems, Japan Marine Science and Technology Center (JAMSTEC), Yokosuka 237-0061, Japan

    Takako Sato & Chiaki Kato

Authors
  1. Adriana Molina-Höppner
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  2. Takako Sato
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  3. Chiaki Kato
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  4. Michael G. Gänzle
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  5. Rudi F. Vogel
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Corresponding author

Correspondence to Michael G. Gänzle.

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Communicated by K. Horikoshi

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Molina-Höppner, A., Sato, T., Kato, C. et al. Effects of pressure on cell morphology and cell division of lactic acid bacteria. Extremophiles 7, 511–516 (2003). https://doi.org/10.1007/s00792-003-0349-0

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  • DOI: https://doi.org/10.1007/s00792-003-0349-0

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