Abstract
Objectives
The aim of this study was to evaluate the influence of [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC) in the physico-chemical properties, antibacterial activity and cytotoxicity of an experimental resin–based sealant.
Materials and methods
An experimental resin-based sealant was formulated with dimethacrylates and a photoinitiator system. METAC was added at 2.5 wt.% (G2.5%) and 5 wt.% (G5%) into the experimental resin–based sealant, and one group remained without METAC as control (GCTRL). The resin-based sealants were analysed for polymerization behaviour and degree of conversion (DC), Knoop hardness (KHN) and softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle, surface free energy (SFE), immediate and long-term micro-shear bond strength (μ-SBS) and antibacterial activity and cytotoxicity against human keratinocytes.
Results
The experimental resin–based sealants presented different polymerization behaviours without significant differences in the DC (p > 0.05). There was no significant difference for initial KHN (p > 0.05). The ΔKHN ranged from 51.62 (±3.70)% to 62.40 (±4.14)%, with higher values for G5% (p < 0.05). G2.5% and G5% had decreased μ-SBS between immediate and long-term tests (p < 0.05) without significant differences among groups in the immediate and long-term analyses (p > 0.05). There were no significant differences for UTS, contact angle and SFE among groups (p > 0.05). G2.5% and G5% presented immediate and long-term antibacterial activity (p < 0.05) without cytotoxicity compared to GCTRL (p > 0.05).
Conclusion
The addition of METAC provided antibacterial activity to the experimental resin–based sealant.
Clinical relevance
METAC is an effective quaternary ammonium compound as an antibacterial agent for resin-based sealants without cytotoxic effects against human keratinocytes.
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This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.
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Garcia, I.M., Rodrigues, S.B., de Souza Balbinot, G. et al. Quaternary ammonium compound as antimicrobial agent in resin-based sealants. Clin Oral Invest 24, 777–784 (2020). https://doi.org/10.1007/s00784-019-02971-4
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DOI: https://doi.org/10.1007/s00784-019-02971-4