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Immunohistochemical expression of basement membrane proteins of verrucous carcinoma of the oral mucosa

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Abstract

Squamous cell carcinoma (SCC) of the oral cavity is an extremely invasive tumour of stratified squamous epithelium that spreads throughout degradation of the basement membrane (BM) and extra-cellular matrix. Oral verrucous carcinoma (VC) is a rare low-grade variant of oral SCC that penetrates into the subepithelial connective tissue. It also has a different clinical behaviour from classical oral SCC. We investigated the immunohistochemical expression of laminin, laminin-5, collagen IV and fibronectin in VC, severe epithelial dysplasia (SED) and SCC in order to analyse if the patter of these molecules expression contributes to the differences in the biological behaviour of these diseases. The staining pattern of laminin was less intensive in SCC compared with SED and VC, and collagen IV expression was increased in VC compared with SED. Discontinuities of laminin, collagen IV and fibronectin were more evident in SED than in VC. This study indicates that VC has a biological behaviour different from SED or SCC, observable by immunohistochemistry in the BM zone.

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References

  1. Al-Rawi NH, Talabani NG (2008) Squamous cell carcinoma of the oral cavity: a case series analysis of clinical presentation and histological grading of 1, 425 cases from Iraq. Clin Oral Investig 12:15–18

    Article  PubMed  Google Scholar 

  2. Aznavoorian S, Murphy AN, Stetler-Stevenson WG, Liotta LA (1993) Molecular aspects of tumor cell invasion and metastasis. Cancer 71:1368–1383

    Article  PubMed  Google Scholar 

  3. Bosman FT, Havenith MG, Visser R, Cleutjens JP (1992) Basement membranes in neoplasia. Prog Histochem Cytochem 24:1–92

    PubMed  Google Scholar 

  4. d'Ardenne AJ (1989) Use of basement membrane markers in tumour diagnosis. J Clin Pathol 42:449–457

    Article  PubMed  Google Scholar 

  5. Driemel O, Dahse R, Berndt A, Pistner H, Hakim SG, Zardi L, Reichert TE, Kosmehl H (2007) High-molecular tenascin-C as an indicator of atypical cells in oral brush biopsies. Clin Oral Investig 11:93–99

    Article  PubMed  Google Scholar 

  6. Eberle C, Phillips JL, Tary P, Menck HR (1997) Quality management in the National Cancer Data Base: a re-abstracting study of the Midwest region. J Regist Manage 24:93–97

    Google Scholar 

  7. Garzino-Demo P, Carrozzo M, Trusolino L et al (1998) Altered expression of alpha 6 integrin subunit in oral squamous cell carcinoma and oral potentially malignant lesions. Oral Oncol 34:204–210

    Article  PubMed  Google Scholar 

  8. Harada T, Shinohara M, Nakamura S, Oka M (1994) An immunohistochemical study of the extracellular matrix in oral squamous cell carcinoma and its association with invasive and metastatic potential. Virchows Arch 424:257–266

    Article  PubMed  Google Scholar 

  9. Jiang L, Wang S, Chen X (2001) Immunohistochemical and ultrastructural study of basement membrane in oral verrucous carcinoma. Zhonghua Kou Qiang Yi Xue Za Zhi 36:308–310

    PubMed  Google Scholar 

  10. Kamboi M, Mahajan S (2007) Micronucleus—an upcoming marker of genotoxic damage. Clin Oral Investig 11:121–126

    Article  Google Scholar 

  11. Kang CJ, Chang JT, Chen TM, Chen IH, Liao CT (2003) Surgical treatment of oral verrucous carcinoma. Chang Gung Med J 26:807–812

    PubMed  Google Scholar 

  12. Kannan S, Balaram P, Chandran GJ et al (1994) Alterations in expression of basement membrane proteins during tumour progression in oral mucosa. Histopathology 24:531–537

    Article  PubMed  Google Scholar 

  13. Kobayashi H, Sagara J, Masumoto J et al (2003) Shifts in cellular localization of moesin in normal oral epithelium, oral epithelial dysplasia, verrucous carcinoma and oral squamous cell carcinoma. J Oral Pathol & Med 32:344–349

    Article  Google Scholar 

  14. Koch BB, Trask DK, Hoffman HT et al (2001) National survey of head and neck verrucous carcinoma: patterns of presentation, care, and outcome. Cancer 92:110–120

    Article  PubMed  Google Scholar 

  15. Impola U, Uitto VJ, Hietanen J et al (2004) Differential expression of matrilysin-1 (MMP-7), 922 kD gelatinase (MMP-9), and metalloelastase (MMP-12) in oral verrucous and squamous cell cancer. J Pathol 202:14–22

    Article  PubMed  Google Scholar 

  16. Leblond CP, Inoue S (1989) Structure, composition, and assembly of basement membrane. Am J Anat 185:367–390

    Article  PubMed  Google Scholar 

  17. Mauch C, Krieg T, Bauer EA (1994) Role of the extracellular matrix in the degradation of connective tissue. Arch Dermatol Res 287:107–114

    Article  PubMed  Google Scholar 

  18. Neville BW, Day TA (2002) Oral cancer and precancerous lesions. CA Cancer J Clin 52:195–215

    Article  PubMed  Google Scholar 

  19. Ogawa A, Fukuta Y, Nakajima T et al (2004) Treatment results of oral verrucous carcinoma and its biological behavior. Oral Oncol 40:793–797

    Article  PubMed  Google Scholar 

  20. Ono Y, Nakanishi Y, Ino Y et al (1999) Clinocopathologic significance of laminin-5 gamma2 chain expression in squamous cell carcinoma of the tongue: immunohistochemical analysis of 67 lesions. Cancer 85:2315–2321

    Article  PubMed  Google Scholar 

  21. Pindborg JJ, Reichart PA, Smith CJ, Van der Waal I (1997) Histological typing of cancer and precancer of the oral mucosa. Springer, Berlin

    Google Scholar 

  22. Poh CF, Zhang L, Lam WL et al (2001) A high frequency of allelic loss in oral verrucous lesions may explain malignant risk. Lab Invest 81:629–634

    PubMed  Google Scholar 

  23. Prioleau PG, Santa Cruz DJ, Meyer JS, Bauer WC (1980) Verrucous carcinoma: a light and electron microscopic, autoradiographic, and immunofluorescence study. Cancer 45:2849–2857

    Article  PubMed  Google Scholar 

  24. Pyke C, Romer J, Kallunki P et al (1994) The gamma 2 chain of kalinin/laminin 5 is preferentially expressed in invading malignant cells in human cancers. Am J Pathol 145:782–791

    PubMed  Google Scholar 

  25. Sakr WA, Zarbo RJ, Jacobs JR, Crissman JD (1987) Distribution of basement membrane in squamous cell carcinoma of the head and neck. Hum Pathol 18:1043–1050

    Article  PubMed  Google Scholar 

  26. Shinohara M, Nakamura S, Harada T, Shimada M, Oka M (1996) Mode of tumor invasion in oral squamous cell carcinoma: improved grading based on immunohistochemical examination of extracellular matrices. Head Neck 18:153–159

    Article  PubMed  Google Scholar 

  27. Sobin LH, Wittekind CH (1997) TNM classification of malignant tumours, 5th edn. Wiley-Liss, New York

    Google Scholar 

  28. Speight PM (2007) Update on oral epithelial dysplasia and progression to cancer. Head and Neck Pathol 1:61–66

    Article  Google Scholar 

  29. Timpl R, Brown JC (1996) Supramolecular assembly of basement membranes. Bioessays 18:123–132

    Article  PubMed  Google Scholar 

  30. Tosios KI, Kapranos N, Papanicolaou SI (1998) Loss of basement membrane components laminin and type IV collagen parallels the progression of oral epithelial neoplasia. Histopathology 33:261–268

    Article  PubMed  Google Scholar 

  31. Worl Health Organization Classification of Tumours (2005) Pathology & genetics. Head and neck tumours. International Agency for Research on Cancer (IARC). In: Barnes L, Eveson JW, Reichart P, Sidransky D (eds) Head and neck tumours. IARC, Lyon, pp 177–180

    Google Scholar 

  32. Yeh CJ (2003) Treatment of verrucous hyperplasia and verrucous carcinoma by shave excision and simple cryosurgery. Int J Oral Maxillofac Surg 32:280–283

    Article  PubMed  Google Scholar 

  33. Yoshimura Y, Mishima K, Obara S et al (2001) Treatment modalities for oral verrucous carcinomas and their outcomes: contribution of radiotherapy and chemotherapy. Int J Clin Oncol 6:192–200

    Article  PubMed  Google Scholar 

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Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Otorhinolaryngology, Department of Clinical Physiopathology, Oral Medicine Section, University of Turin, Unito Lingotto Dental Institute c/o Lingotto, Via Nizza 230, 10126, Turin, Italy

    Paolo G. Arduino, Marco Pagano, Roberto Broccoletti & Sergio Gandolfo

  2. Department of Oral Medicine, School of Dental Sciences, University of Newcastle upon Tyne, Newcastle upon Tyne, UK

    Marco Carrozzo

  3. Eastman Dental Institute, University College of London, London, UK

    Crispian Scully

Authors
  1. Paolo G. Arduino
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  2. Marco Carrozzo
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  3. Marco Pagano
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  4. Roberto Broccoletti
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  5. Crispian Scully
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  6. Sergio Gandolfo
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Correspondence to Paolo G. Arduino.

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Arduino, P.G., Carrozzo, M., Pagano, M. et al. Immunohistochemical expression of basement membrane proteins of verrucous carcinoma of the oral mucosa. Clin Oral Invest 14, 297–302 (2010). https://doi.org/10.1007/s00784-009-0296-y

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  • DOI: https://doi.org/10.1007/s00784-009-0296-y

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