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Experimental study of calcium phosphate cement impregnated with dideoxy-kanamycin B

  • Original article
  • Published:
Journal of Orthopaedic Science

Abstract

Background

The present study was undertaken to examine whether antibiotic-impregnated calcium phosphate cement (CPC) would provide a valid means of treating osteomyelitis.

Methods

The antibiotic used for the impregnation was dideoxy-kanamycin B (DKB), which is available in two forms (powder and liquid). Columnar test specimens (diameter 7 mm, height 14 mm) were prepared by adding the liquid or powdered DKB.

Group A: Three types (6.25-titer, 12.5-titer, 25-titer) of test specimen were prepared by mixing the setting solution and DKB solutions into cement.

Group B: Three types (25-titer, 50-titer, 100-titer) of test specimen were prepared by mixing the setting solution and DKB powder into cement.

Group C: A control specimen was prepared by mixing the setting solution into the cement. The study included a consistency test, setting-time test, compressive strength test, porosity test, and elution test.

Results

The value for the consistency test was >23 mm in all test groups. The results of the setting-time test showed that the setting time became significantly longer as the DKB content increased for groups A group B. Compressive strength decreased as the antibiotic content increased, although all specimens remained sufficiently strong for clinical application. In group A the porosity did not differ significantly depending on the antibiotic content, whereas in group B the porosity increased significantly as the antibiotic content increased. In the elution test using specimens with the same titer (25 titer), the elution efficiency was higher in group A than in group B, and the duration of elution was longer in group A.

Conclusions

Although polymethylmethacrylate (PMMA) has been conventionally used as a drug-delivery system (DDS), the results of the present study indicate that CPC shows better elution efficiency than PMMA. It is thus a promising DDS for the treatment of osteomyelitis.

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References

  1. N Hidaka Y Yamano Y Kadoya N Nishimura (2002) ArticleTitleCalcium phosphate bone cement for treatment of distal radius fractures: a preliminary report J Orthop Sci 7 182–7 10.1007/s007760200031 Occurrence Handle10.1007/s007760200031 Occurrence Handle11956977

    Article  PubMed  Google Scholar 

  2. K Kita K Masada M Yasuda E Takeuchi (1998) ArticleTitleEnchondroma protuberance of the phalanx: a case report J Hand Surg [Am] 28 1052–4 10.1016/S0363-5023(03)00486-6 Occurrence Handle10.1016/S0363-5023(03)00486-6

    Article  Google Scholar 

  3. Y Matsuyama M Goto H Yoshihara T Tsuji Y Sakai H Nakamura et al. (2004) ArticleTitleVertebral reconstruction with biodegradable calcium phosphate cement in the treatment of osteoporotic vertebral compression fracture using instrumentation J Spinal Disord Tech 17 291–6 10.1097/01.bsd.0000097253.54459.a6 Occurrence Handle10.1097/01.bsd.0000097253.54459.a6 Occurrence Handle15280757

    Article  PubMed  Google Scholar 

  4. T Sasaki Y Ishibashi H Katano A Nagumo S Toh (2005) ArticleTitleIn vitro elution of vancomycin from calcium phosphate cement J Arthroplasty 20 1055–9 10.1016/j.arth.2005.03.035 Occurrence Handle10.1016/j.arth.2005.03.035 Occurrence Handle16376263

    Article  PubMed  Google Scholar 

  5. M Suzuki T Tsukeoka Y Tsuneizumi T Kim H Yamanaka H Nakamura et al. (2004) ArticleTitleMechanical strength and in vitro antibiotic release profile of antibiotics-loaded calcium phosphate bone paste Rinshou Seikeigeka (Clin Orthop) 39 309–14

    Google Scholar 

  6. K Kurashima H Kurita M Hirano JMA de Blieck CPAT Klein K De Groot (1995) ArticleTitleCalcium phosphate cement: in vitro and in vivo studies of the α-tricalcium phosphate-dicalcium phosphate dibasic-tetracalcium phosphate monoxide system J Mater Sci Mater Med 6 340–7 10.1007/BF00120302 Occurrence Handle10.1007/BF00120302

    Article  Google Scholar 

  7. N Asaoka M Misago M Hirano H Takeuchi (1999) ArticleTitleMechanical and chemical properties of the injectable calcium phosphate cement Bioceramics 12 525–8 Occurrence Handle1:CAS:528:DC%2BD3cXkslCltQ%3D%3D

    CAS  Google Scholar 

  8. T Kokubo T Hayashi S Sakka T Kitsugi T Yamamuro (1987) ArticleTitleBonding between bioactive glasses, glass-ceramics or ceramics in a simulated body fluid Yogyo Kyokai Shi 95 785–91 Occurrence Handle1:CAS:528:DyaL2sXls1WlsLc%3D

    CAS  Google Scholar 

  9. HW Buchholz H Engelbrecht (1970) ArticleTitleÜber die depotwirkung einiger Antibiotika bei Vermischung mit dem Kunstharz Palacos Chirurg 40 511–5

    Google Scholar 

  10. PD Levin (1975) ArticleTitleThe effectiveness of various antibiotics in methylmethacrylate J Bone Joint Surg Br 57 234–7 Occurrence Handle1:STN:280:DyaE2M7ovFGksA%3D%3D Occurrence Handle167026

    CAS  PubMed  Google Scholar 

  11. MW Chapman K Hadley (1976) ArticleTitleThe effect of polymethylmethacrylate and antibiotic combinations on bacterial viability: an in vitro and preliminary in vivo study J Bone Joint Surg Am 58 76–81 Occurrence Handle1:STN:280:DyaE287itVKmtw%3D%3D Occurrence Handle765348

    CAS  PubMed  Google Scholar 

  12. KE Marks CL Nelson EP Lautenschlager (1976) ArticleTitleAntibiotic-impregnated acrylic bone cement J Bone Joint Surg Am 58 358–64 Occurrence Handle1:STN:280:DyaE287mslKksA%3D%3D Occurrence Handle770477

    CAS  PubMed  Google Scholar 

  13. B Picknell L Mizen R Sutherland (1977) ArticleTitleAntibacterial activity of antibiotics in acrylic bone cement J Bone Joint Surg Br 59 302–7 Occurrence Handle1:STN:280:DyaE2s3kvFOguw%3D%3D Occurrence Handle408356

    CAS  PubMed  Google Scholar 

  14. E Ger D Dall T Miles A Forder (1977) ArticleTitleBone cement and antibiotics S Afr Med J 51 276–9 Occurrence Handle1:STN:280:DyaE2s7ltFaisQ%3D%3D Occurrence Handle847587

    CAS  PubMed  Google Scholar 

  15. K Nampei (1979) ArticleTitleExperimental and clinical studies on the antibacterial activities of acrylic bone cement with antibiotics Osakashi Igakukai Zasshi (J Osaka City Med Cent) 28 573–95

    Google Scholar 

  16. I Kinoshita (1973) ArticleTitlePreventive measures against postoperative infection following prosthetic arthroplasty: study of bone cement mixed with antibiotics Chuubu Nihon Seikeigeka Saigaigeka Gakkai Zasshi (Cent Jpn J Orthop Traumatol) 16 899–915

    Google Scholar 

  17. K Kanellakopoulou EJ Gimarellos-Bourboulis (2000) ArticleTitleCarrier systems for the local delivery of antibiotics in bone infections Drugs 59 1223–32 10.2165/00003495-200059060-00003 Occurrence Handle10.2165/00003495-200059060-00003 Occurrence Handle1:CAS:528:DC%2BD3cXltFOgt7k%3D Occurrence Handle10882159

    Article  CAS  PubMed  Google Scholar 

  18. Y Shinto A Uchida F Korkusuz N Araki K Ono (1992) ArticleTitleCalcium hydroxyapatite ceramic used as a delivery system for antibiotics J Bone Joint Surg Br 74 600–4 Occurrence Handle1:STN:280:DyaK38zisFWnsg%3D%3D Occurrence Handle1320622

    CAS  PubMed  Google Scholar 

  19. M Itokazu W Yang T Aoki A Ohara N Kato (1998) ArticleTitleSynthesis of antibiotic-loaded interporous hydroxyapatite blocks by vacuum method and in vitro drug release testing Biomaterials 19 817–9 10.1016/S0142-9612(97)00237-8 Occurrence Handle10.1016/S0142-9612(97)00237-8 Occurrence Handle1:CAS:528:DyaK1cXjvVantbs%3D Occurrence Handle9663758

    Article  CAS  PubMed  Google Scholar 

  20. C Hamanishi K Kitamoto S Tanaka M Otsuka Y Doi T Kitahashi (1996) ArticleTitleA self-setting TTCP-DCPD apatite cement for release of vancomycin J Biomed Mater Res 33 139–43 10.1002/(SICI)1097-4636(199623)33:3<139::AID-JBM3>3.0.CO;2-R Occurrence Handle10.1002/(SICI)1097-4636(199623)33:3<139::AID-JBM3>3.0.CO;2-R Occurrence Handle1:CAS:528:DyaK28Xlt1yhtLw%3D Occurrence Handle8864885

    Article  CAS  PubMed  Google Scholar 

  21. M Hasegawa A Sudo VS Komlev SM Barinov A Uchida (2004) ArticleTitleHigh release of antibiotic from a novel hydroxyapatite with bimodal pore size distribution J Biomed Mater Res B Appl Biomater 70 332–9 10.1002/jbm.b.30047 Occurrence Handle10.1002/jbm.b.30047 Occurrence Handle15264316

    Article  PubMed  Google Scholar 

  22. A Tanaka Y Ishii (2002) ArticleTitleMechanical properties of the femur after injection of calcium phosphate cement containing arbekacin sulfate and polylactic acid in a rat model of experimental osteoporosis J Orthop Sci 7 370–8 10.1007/s007760200062 Occurrence Handle10.1007/s007760200062 Occurrence Handle1:CAS:528:DC%2BD38XlvFOmtLk%3D Occurrence Handle12077664

    Article  CAS  PubMed  Google Scholar 

  23. H Umezawa S Umezawa T Tsuchiya Y Okazaki (1971) ArticleTitle3′,4′-Dideoxy-kanamycin B active against kanamycin-resistant Escherichia coli and Pseudomonas aeruginosa J Antibiot (Tokyo) 24 485–7 Occurrence Handle1:CAS:528:DyaE38XktVWiurs%3D

    CAS  Google Scholar 

  24. L Røhl E Larsen F Linde A Odgaard J Jørgensen (1991) ArticleTitleTensile and compressive properties of cancellous bone J Biomech 24 1143–9 10.1016/0021-9290(91)90006-9 Occurrence Handle10.1016/0021-9290(91)90006-9 Occurrence Handle1769979

    Article  PubMed  Google Scholar 

  25. DR Carter DM Spengler (1978) ArticleTitleMechanical properties and composition of cortical bone Clin orthop 135 192–217 Occurrence Handle361320

    PubMed  Google Scholar 

  26. S Nakazawa M Otsuki M Nakao S Tsukazawa H Naha K Futaki (1974) ArticleTitleBacteriological studies on 3′,4′-dideoxykanamycin B Chemotherapy 22 779–84 Occurrence Handle1:CAS:528:DyaE2MXhtFKnsQ%3D%3D

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Yoshimoto Orthopaedic Surgery and Surgical Hospital, 136 Noguchi, Yamatotakada, Nara, 635-0075, Japan

    Osamu Kisanuki

  2. Department of Orthopaedic Surgery, Nara Medical University, Nara, Japan

    Hiroshi Yajima & Yoshinori Takakura

  3. Department of Precision Engineering, Graduate school of Engineering, The University of Tokyo, Tokyo, Japan

    Tomohiro Umeda

Authors
  1. Osamu Kisanuki
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  2. Hiroshi Yajima
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  3. Tomohiro Umeda
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  4. Yoshinori Takakura
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Kisanuki, O., Yajima, H., Umeda, T. et al. Experimental study of calcium phosphate cement impregnated with dideoxy-kanamycin B. J Orthop Sci 12, 281–288 (2007). https://doi.org/10.1007/s00776-007-1124-3

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  • DOI: https://doi.org/10.1007/s00776-007-1124-3

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