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Binding and interaction of di- and tri-substituted organometallic triptycene palladium complexes with DNA

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Abstract

Two triptycene-based ligands with pendant bromophenyl units have been prepared. These triptycene derivatives have been used as synthons for the synthesis of di and tri nuclear palladium complexes. The organic molecules and their corresponding organometallic complexes have been fully characterized using nuclear magnetic resonance (NMR), infrared (IR) spectroscopy and mass spectrometry. The mode of binding and effect of the complexes on pUC19 plasmid, calf thymus DNA and oligomer duplex DNA have been investigated by a host of analytical methods. The complexes brought about unwinding of supercoiled plasmid and the unwinding angle was found to be related to the binding affinity of the complexes with DNA, where both these parameters were guided by the structure of the complexes. Concentration-dependent inhibition of endonuclease activity of SspI and BamHI by the complexes indicates preference for G/C sequence for binding to DNA. However, neither the complexes did not introduce any cleavage at abasic site in oligomer duplex DNA, nor they created linear form of the plasmid upon co-incubation with the DNA samples. The interactions of the complexes with DNA were found to be strongly guided by the structure of the complexes, where intercalation as well as groove binding was observed, without inflicting any damage to the DNA. The mode of interaction of the complexes with DNA was further confirmed by isothermal calorimetry.

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Acknowledgments

This work was supported by a Grant to P. D. and N. D from Department of Biotechnology (DBT), Govt. of India, Project No. BT/PR3444/NNT/28/560/2011. R. K. and S. B. are thankful to IIT Patna for fellowship. Authors are thankful to Prof. P. K. Das of IACS, Kolkata for help with CD facility. All the authors gratefully acknowledge the availability of Infrastructural and experimental facilities provided by IIT Patna. The authors also acknowledge SID, IISc Bangalore and SAIF, Punjab University for analytical facilities.

Author contribution statement

All authors have contributed to the idea, the designing and planning of the studies as well as interpretation and discussion of the results. S.B. synthesized the triptycene based organic ligands and their corresponding organometallic complexes. R.K. carried out the experiments related to evaluation of biological activity of triptycene based molecules reported herein. All authors have contributed to compiling the manuscript and have approved the final manuscript.

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Authors and Affiliations

  1. Department of Chemistry, Indian Institute of Technology Patna, Patliputra Colony, Patna, 800013, Bihar, India

    Rina Kumari, Sourav Bhowmick, Neeladri Das & Prolay Das

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  1. Rina Kumari
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  2. Sourav Bhowmick
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  3. Neeladri Das
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  4. Prolay Das
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Correspondence to Neeladri Das or Prolay Das.

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Kumari, R., Bhowmick, S., Das, N. et al. Binding and interaction of di- and tri-substituted organometallic triptycene palladium complexes with DNA. J Biol Inorg Chem 19, 1221–1232 (2014). https://doi.org/10.1007/s00775-014-1180-z

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