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Siderophore-mediated iron transport in Bacillus subtilis and Corynebacterium glutamicum

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Abstract

Hexadentate bacillibactin is the siderophore of Bacillus subtilis and is structurally similar to the better known enterobactin of Gram-negative bacteria such as Escherichia coli. Although both are triscatecholamide trilactones, the structural differences of these two siderophores result in opposite metal chiralities, different affinity for ferric ion, and dissimilar iron transport behaviors. Bacillibactin was first reported as isolated from Corynebacterium glutamicum and called corynebactin. However, failure of iron-starved C. glutamicum to transport 55Fe bacillibactin and lack of required bacillibactin biosynthetic genes suggest that bacillibactin is not the siderophore produced by this organism. Iron transport mediated by siderophores in B. subtilis occurs through a transport process that is specific for the iron chelating moiety, with parallel pathways for catecholates and hydroxamates. For bacillibactin, enterobactin, and their analogs, neither chirality nor presence of an amino acid spacer affects the uptake and transport process, but alteration of the net charge and size of the molecule impedes the recognition.

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Acknowledgements

Lisa Whitworth assisted in maintenance of the bacterial cultures, and we thank John Helmann, Rebecca Abergel, and Trisha Hoette for many helpful discussions. This work was supported by NIH grant AI 11744.

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Authors and Affiliations

  1. Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720-1460, USA

    Emily A. Dertz & Kenneth N. Raymond

  2. Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 451 Smythe Road, Ottawa, ON, Canada, K1H 8M5

    Alain Stintzi

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  1. Emily A. Dertz
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  2. Alain Stintzi
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Correspondence to Kenneth N. Raymond.

Additional information

Paper number 77 in the series Coordination Chemistry of Microbial Iron Transport Compounds. See Abergel et al. [1].

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Dertz, E.A., Stintzi, A. & Raymond, K.N. Siderophore-mediated iron transport in Bacillus subtilis and Corynebacterium glutamicum . J Biol Inorg Chem 11, 1087–1097 (2006). https://doi.org/10.1007/s00775-006-0151-4

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  • DOI: https://doi.org/10.1007/s00775-006-0151-4

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