Abstract
The aim of the present study was to examine whether a holistic approach combining nutrition and lifestyle counselling with the consumption of milk and yoghurt enriched with calcium, vitamin D3 and phylloquinone (vitamin K1) or menaquinone (vitamin K2) would have any additional benefit on bone mineral density (BMD) indices measured at various skeletal sites using two different techniques, dual energy X-ray absorptiometry and quantitative ultrasonography (QUS). A sample of 115 postmenopausal women were randomized to three intervention groups, receiving daily via fortified milk and yoghurt and for 12 months, 800 mg calcium and 10 μg vitamin D3 (CaD group, n = 26); 800 mg calcium, 10 μg vitamin D3 and 100 μg vitamin K1 (CaDK1 group, n = 26); 800 mg calcium, 10 μg vitamin D3 and 100 μg vitamin K2 (CaDK2 group, n = 24); and a control group (CO group, n = 39) following their usual diet. All three intervention groups attended biweekly nutrition and lifestyle counselling sessions. Total BMD significantly increased in all three intervention groups and these changes were significantly higher compared to the CO (P < 0.001). Furthermore, the significant increases observed for L2–L4 BMD in the CaDK1 and CaDK2 groups were found to be significantly higher compared to the decrease observed in the CO (P = 0.001). No significant differences were observed for QUS parameters. The combined approach used in the current study led to favourable changes for all three intervention groups in total body BMD, while an additional benefit was observed for L2–L4 BMD in CaDK1 and CaDK2 groups. No significant differences were observed among groups in any of the QUS parameters.
References
Harvey N, Dennison E, Cooper C (2010) Osteoporosis: impact on health and economics. Nat Rev Rheumatol 6:99–105
NIH Consensus Conference (1994) Optimal calcium intake. NIH Consensus Development panel on optimal calcium intake. JAMA 272:1942–1948
Mosekilde L (2005) Vitamin D and the elderly. Clin Endocrinol (Oxf) 62:265–281
Calvo MS, Whiting SJ, Barton CN (2005) Vitamin D intake: a global perspective of current status. J Nutr 135:310–316
Rapuri PB, Kinyamu HK, Gallagher JC, Haynatzka V (2002) Seasonal changes in calciotropic hormones, bone markers, and bone mineral density in elderly women. J Clin Endocrinol Metab 87:2024–2032
Iwamoto J, Takeda T, Ichimura S (2001) Effect of menatetrenone on bone mineral density and incidence of vertebral fractures in postmenopausal women with osteoporosis: a comparison with the effect of etidronate. J Orthop Sci 6:487–492
Cheung AM, Tile L, Lee Y, Tomlinson G, Hawker G, Scher J, Hu H, Vieth R, Thompson L, Jamal S, Josse R (2008) Vitamin K supplementation in postmenopausal women with osteopenia (ECKO trial): a randomized controlled trial. PLoS Med 5:e196
Shiraki M, Shiraki Y, Aoki C, Miura M (2000) Vitamin K2 (menatetrenone) effectively prevents fractures and sustains lumbar bone mineral density in osteoporosis. J Bone Miner Res 15:515–521
Ishida Y, Kawai S (2004) Comparative efficacy of hormone replacement therapy, etidronate, calcitonin, alfacalcidol, and vitamin K in postmenopausal women with osteoporosis: the Yamaguchi Osteoporosis Prevention Study. Am J Med 117:549–555
Manios Y, Moschonis G, Trovas G, Lyritis GP (2007) Changes in biochemical indexes of bone metabolism and bone mineral density after a 12-mo dietary intervention program: the Postmenopausal Health Study. Am J Clin Nutr 86:781–789
Chee WS, Suriah AR, Chan SP, Zaitun Y, Chan YM (2003) The effect of milk supplementation on bone mineral density in postmenopausal Chinese women in Malaysia. Osteoporos Int 14:828–834
Prince R, Devine A, Dick I, Criddle A, Kerr D, Kent N, Price R, Randell A (1995) The effects of calcium supplementation (milk powder or tablets) and exercise on bone density in postmenopausal women. J Bone Miner Res 10:1068–1075
Storm D, Eslin R, Porter ES, Musgrave K, Vereault D, Patton C, Kessenich C, Mohan S, Chen T, Holick MF, Rosen CJ (1998) Calcium supplementation prevents seasonal bone loss and changes in biochemical markers of bone turnover in elderly New England women: a randomized placebo-controlled trial. J Clin Endocrinol Metab 83:3817–3825
Schurgers LJ, Teunissen KJ, Hamulyak K, Knapen MH, Vik H, Vermeer C (2007) Vitamin K-containing dietary supplements: comparison of synthetic vitamin K1 and natto-derived menaquinone-7. Blood 109:3279–3283
Janz NK, Becker MH (1984) The health belief model: a decade later. Health Educ Q 11:1–47
Bandura A, Adams NE, Beyer J (1977) Cognitive processes mediating behavioral change. J Pers Soc Psychol 35:125–139
Magkos F, Manios Y, Babaroutsi E, Sidossis LS (2005) Quantitative ultrasound calcaneus measurements: normative data for the Greek population. Osteoporos Int 16:280–288
Institute of Medicine Food and Nutrition Board (2001) Dietary reference intakes: applications in dietary assessment. National Academy Press, Washington
Riggs BL, O’Fallon WM, Muhs J, O’Connor MK, Kumar R, Melton LJ 3rd (1998) Long-term effects of calcium supplementation on serum parathyroid hormone level, bone turnover, and bone loss in elderly women. J Bone Miner Res 13:168–174
Moschonis G, Katsaroli I, Lyritis GP, Manios Y (2010) The effects of a 30-month dietary intervention on bone mineral density: The Postmenopausal Health Study. Br J Nutr. 104: 100–107
Bolton-Smith C, McMurdo ME, Paterson CR, Mole PA, Harvey JM, Fenton ST, Prynne CJ, Mishra GD, Shearer MJ (2007) Two-year randomized controlled trial of vitamin K1 (phylloquinone) and vitamin D3 plus calcium on the bone health of older women. J Bone Miner Res 22:509–519
Cockayne S, Adamson J, Lanham-New S, Shearer MJ, Gilbody S, Torgerson DJ (2006) Vitamin K and the prevention of fractures: systematic review and meta-analysis of randomized controlled trials. Arch Intern Med 166:1256–1261
Schaafsma A, Muskiet FA, Storm H, Hofstede GJ, Pakan I, Van der Veer E (2000) Vitamin D(3) and vitamin K(1) supplementation of Dutch postmenopausal women with normal and low bone mineral densities: effects on serum 25-hydroxyvitamin D and carboxylated osteocalcin. Eur J Clin Nutr 54:626–631
Vermeer C, Shearer MJ, Zittermann A, Bolton-Smith C, Szulc P, Hodges S, Walter P, Rambeck W, Stocklin E, Weber P (2004) Beyond deficiency: potential benefits of increased intakes of vitamin K for bone and vascular health. Eur J Nutr 43:325–335
Schaafsma A, de Vries PJ, Saris WH (2001) Delay of natural bone loss by higher intakes of specific minerals and vitamins. Crit Rev Food Sci Nutr 41:225–249
Moschonis G, Manios Y (2006) Skeletal site-dependent response of bone mineral density and quantitative ultrasound parameters following a 12-month dietary intervention using dairy products fortified with calcium and vitamin D: the Postmenopausal Health Study. Br J Nutr 96:1140–1148
Rosenthall L, Caminis J, Tenehouse A (1999) Calcaneal ultrasonometry: response to treatment in comparison with dual X-ray absorptiometry measurements of the lumbar spine and femur. Calcif Tissue Int 64:200–204
Sahota O, San P, Cawte SA, Pearson D, Hosking DJ (2000) A comparison of the longitudinal changes in quantitative ultrasound with dual-energy X-ray absorptiometry: the four-year effects of hormone replacement therapy. Osteoporos Int 11:52–58
Acknowledgments
The study was supported by a research grant from FrieslandCampina BV. The authors would like to thank Anastasia Vandorou, Katerina Kyriakou, Vasiliki Dede, Odysseas Androutsos, Konstantina Maragkopoulou, Efstathoula Argyri, Angeliki Giannopoulou, dieticians, for their valuable contribution in data collection and processing.
Conflict of interest
YM also works as a part-time science and nutrition consultant for FrieslandCampina Hellas. AS also works as a cluser leader Nutrition for FrieslandCampina BV. The study sponsor had no role in the collection and analysis of the data, the writing of the manuscript, and the submission of the paper. None of the other authors had any potential conflict of interest.
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All authors contributed in the study design, writing and revising the manuscript; GM, SK and YM were responsible for data collection, management and statistical analyses.
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Moschonis, G., Kanellakis, S., Papaioannou, N. et al. Possible site-specific effect of an intervention combining nutrition and lifestyle counselling with consumption of fortified dairy products on bone mass: the Postmenopausal Health Study II. J Bone Miner Metab 29, 501–506 (2011). https://doi.org/10.1007/s00774-010-0256-2
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DOI: https://doi.org/10.1007/s00774-010-0256-2