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Dietary l-proline supplementation confers immunostimulatory effects on inactivated Pasteurella multocida vaccine immunized mice

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Abstract

This study was conducted to determine the immunostimulatory effect of l-proline on inactivated vaccine immunized mice. Ninety-five female KM mice were randomly divided into five groups: (1) mice received dietary supplementation with 0.4 % l-proline and immunized with inactivated vaccine (V–P group); (2) mice received dietary supplementation with 0.3 % l-alanine (isonitrogenous control) and immunized with inactivated vaccine (V–A group, negative control); (3) mice were immunized with inactivated vaccine with oil adjuvant (V–O group, positive control); (4) mice were immunized with inactivated vaccine with aluminum hydroxide adjuvant (V–H group, positive control); (5) mice immunized with phosphate-buffered saline (control group). All mice were dead in the control group between 36 and 48 h post infection. Mice in the V–P group showed 100 % protection after challenge with P. multocida serotype A (CQ2) at dose of 4.4 × 105 CFU (2LD50). Meanwhile, serum antibody titers in the V–P group were higher than those in the V–A group before infection and those in the V–A and V–O groups at 36 h post infection. Moreover, serum IL-1β levels in the V–P group were lower than those in V–O group. Furthermore, serum GSH-PX levels in the V–P group were higher than those in the V–A and V–O groups. Collectively, dietary proline supplementation confers beneficial immunostimulatory effects in inactivated P. multocida vaccine immunized mice.

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Abbreviations

BSA:

Bovine serum albumin

GSH-PX:

Glutathione peroxidase

IL:

Interleukin

PBS:

Phosphate-buffered saline

TNF-α:

Tumor necrosis factor alpha

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Acknowledgments

This research was jointly supported by MATS-Beef Cattle Yak system(CARS-38), National Basic Research Program of China (2012CB124704, 2013CB127301), National Natural Science Foundation of China (31110103909,30901040, 31272463, 30928018, and 31101729), the State Key Laboratory of Food Science and Technology, the Nanchang University project (SKLF-TS-201108, SKLF-KF-201005, SKLF-KF-201216), the CAS/SAFEA International Partnership Program for Creative Research Teams, Chinese Universities Scientific Funds (2012RC024), and the Thousand-People-Talent program at China Agricultural University, Texas AgriLife Research project (H-8200).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Agroecology and Processing of Subtropical Region, Institute of Subtropical Agriculture, Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center for Animal and Poultry Science, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central China, The Chinese Academy of Science, Ministry of Agriculture, 410125, Changsha City, Hunan, People’s Republic of China

    Wenkai Ren, Gang Liu, Yulong Yin, Tiejun Li & Guoyao Wu

  2. Chongqing Key Laboratory of Forage and Herbivores, College of Animal Science and Technology, Southwest University, 400716, Chongqing, China

    Wenkai Ren, Lingxiu Zou, Nengzhang Li, Yan Wang & Yuanyi Peng

  3. State Key Laboratory of Food Science and Technology, Nanchang University, 330047, Nanchang, China

    Zheng Ruan & Yulong Yin

  4. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, 430023, Wuhan, China

    Yongqing Hou

  5. State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, China

    Guoyao Wu

  6. Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA

    Guoyao Wu

Authors
  1. Wenkai Ren
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  2. Lingxiu Zou
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  3. Zheng Ruan
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  4. Nengzhang Li
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  5. Yan Wang
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  6. Yuanyi Peng
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  7. Gang Liu
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  8. Yulong Yin
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  9. Tiejun Li
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  10. Yongqing Hou
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  11. Guoyao Wu
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Corresponding authors

Correspondence to Yuanyi Peng, Yulong Yin or Tiejun Li.

Additional information

W. Ren and L. Zou contributed equally to this study.

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Ren, W., Zou, L., Ruan, Z. et al. Dietary l-proline supplementation confers immunostimulatory effects on inactivated Pasteurella multocida vaccine immunized mice. Amino Acids 45, 555–561 (2013). https://doi.org/10.1007/s00726-013-1490-4

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  • DOI: https://doi.org/10.1007/s00726-013-1490-4

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