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Peptides on the Surface: Spin-Label EPR and PELDOR Study of Adsorption of the Antimicrobial Peptides Trichogin GA IV and Ampullosporin A on the Silica Nanoparticles

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Abstract

The properties of antimicrobial peptides adsorbed on inorganic or organic surfaces are of interest for their potential applications in intracellular drug delivery. In this work, continuous-wave (CW) electron paramagnetic resonance (EPR) and pulsed electron-electron double resonance (PELDOR) techniques were applied to study adsorption of the short-sequence trichogin GA IV and the medium-length sequence ampullosporin A antimicrobial peptides on the monodisperse colloidal silica nanospheres of 20 nm diameter. The results obtained by CW EPR support the view that the adsorbed peptides form close-packed clusters. PELDOR data show that both trichogin and ampullosporin adsorbed on the silica surface possess a more disordered conformation as compared to that in solution. For ampullosporin, disordering is much more pronounced than for trichogin. After desorption, the peptides restored their conformations; upon adsorption the peptides in some cases may lose partly their biradical character.

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Acknowledgments

We thank Dr. A.D. Milov for helpful discussion and assistance in some of the measurements. This work was supported by the Russian Science Foundation, project no. 15-15-00021.

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Authors and Affiliations

  1. Institute of Chemical Kinetics and Combustion, RAS, Novosibirsk, 630090, Russian Federation

    Victoria N. Syryamina, Rimma I. Samoilova, Yuri D. Tsvetkov & Sergei A. Dzuba

  2. Novosibirsk State University, Novosibirsk, 630090, Russian Federation

    Victoria N. Syryamina, Arkady V. Ischenko & Sergei A. Dzuba

  3. Boreskov Institute of Catalysis, RAS, Novosibirsk, 630090, Russian Federation

    Arkady V. Ischenko

  4. Department of Chemical Sciences, University of Padova, 35131, Padua, Italy

    Marta De Zotti, Marina Gobbo, Claudio Toniolo & Fernando Formaggio

  5. Institute of Biomolecular Chemistry, Padova Unit, CNR, 35131, Padua, Italy

    Marina Gobbo, Claudio Toniolo & Fernando Formaggio

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  1. Victoria N. Syryamina
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  2. Rimma I. Samoilova
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  3. Yuri D. Tsvetkov
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  4. Arkady V. Ischenko
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  5. Marta De Zotti
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  6. Marina Gobbo
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  9. Sergei A. Dzuba
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Correspondence to Sergei A. Dzuba.

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Syryamina, V.N., Samoilova, R.I., Tsvetkov, Y.D. et al. Peptides on the Surface: Spin-Label EPR and PELDOR Study of Adsorption of the Antimicrobial Peptides Trichogin GA IV and Ampullosporin A on the Silica Nanoparticles. Appl Magn Reson 47, 309–320 (2016). https://doi.org/10.1007/s00723-015-0745-5

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  • DOI: https://doi.org/10.1007/s00723-015-0745-5

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