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Stress-induced Oryza sativa BAT1 dual helicase exhibits unique bipolar translocation

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Abstract

HLA-B associated transcript 1 (BAT1) protein, also named as spliceosome RNA helicase UAP56, is a member of the DExD/H-box family of helicases. However, regulation under stress, biochemical properties, and functions of plant homologue of BAT1 are poorly understood. Here, we report the purification and detailed biochemical characterization of the Oryza sativa homologue of BAT1 (OsBAT1/UAP56) protein (52 kDa) and regulation of its transcript under abiotic stress. OsBAT1 transcript levels are enhanced in rice seedlings in response to abiotic stress including salt stress and abscisic acid. Purified OsBAT1 protein exhibits the DNA- and RNA-dependent ATPase, RNA helicase, and DNA- and RNA-binding activities. Interestingly OsBAT1 also exhibits unique DNA helicase activity, which has not been reported so far in any BAT1 homologue. Moreover, OsBAT1 translocates in both the 3′ to 5′ and 5′ to 3′ directions, which is also a unique property. The K m value for OsBAT1 DNA helicase is 0.9753 nM and for RNA helicase is 1.7536 nM, respectively. This study demonstrates several unique characteristics of OsBAT1 especially its ability to unwind both DNA and RNA duplexes; bipolar translocation and its transcript upregulation under abiotic stresses indicate that it is a multifunctional protein. Overall, this study represents significant contribution in advancing our knowledge regarding functions of OsBAT1 in RNA and DNA metabolism and its putative role in abiotic stress signaling in plants.

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Acknowledgements

The work on helicases in N. T.’s laboratory is partially supported by Department of Science and Technology (DST) and Department of Biotechnology (DBT), Government of India.

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There is no conflict of interest.

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Authors and Affiliations

  1. International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Narendra Tuteja, Mohammed Tarique, Dipesh Kumar Trivedi, Ranjan Kumar Sahoo & Renu Tuteja

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  1. Narendra Tuteja
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  2. Mohammed Tarique
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  3. Dipesh Kumar Trivedi
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  4. Ranjan Kumar Sahoo
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  5. Renu Tuteja
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Correspondence to Narendra Tuteja.

Additional information

Handling Editor: Bhumi Nath Tripathi

Gene Bank Accession Number of OsBAT1: GQ 478227 (Locus id: LOC_Os01g36890.2)

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Supplementary Figure S1

Alignment of OsBAT1 protein. An alignment of amino acid sequences using the NCBI database revealed that OsBAT1 aligned contiguously and showed highest homology with its counterpart UAP56 from Arabidopsis thaliana (~89 %). (PPTX 146 kb)

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Tuteja, N., Tarique, M., Trivedi, D.K. et al. Stress-induced Oryza sativa BAT1 dual helicase exhibits unique bipolar translocation. Protoplasma 252, 1563–1574 (2015). https://doi.org/10.1007/s00709-015-0791-8

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