Abstract
Introgression and functional expression of either the PcINO1 (l-myo-inositol 1-phosphate synthase or MIPS coding gene from the wild halophytic rice, Porteresia coarctata) or McIMTI (inositol methyl transferase, IMTI coding gene from common ice plant Mesembryanthemum crystallinum) has earlier been shown to confer salt tolerance to transgenic tobacco plants (Sheveleva et al., Plant Physiol 115:1211–1219, 1997; Majee et al., J Biol Chem 279:28539–28552, 2004). In this communication, we show that transgenic tobacco plants co-expressing PcINO1 and McIMT1 gene either in cytosol or in chloroplasts accumulate higher amount of total inositol (free and methyl inositol) compared to non-transgenic plants. These transgenic plants were more competent in terms of growth potential and photosynthetic activity and were less prone to oxidative stress under salt stress. A positive correlation between the elevated level of total inositol and methylated inositol and the capability of the double transgenic plants to withstand a higher degree of salt stress compared to the plants expressing either PcINO1 or McIMT1 alone is inferred.
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References
Adams M, Richter A, Hill AK, Colmer TD (2005) Salt tolerance in Eucalyptus sp: identity and response of putative osmolytes. Plant Cell Environ 28:772–787
Allakhverdiev SI, Nishiyama Y, Miyairi S, Yamamoto H, Inagaki N, Kanesaki Y, Murata N (2002) Salt stress inhibits the repair of photodamaged photosystem II by suppressing the transcription and translation of psbA genes in Synechocystis1. Plant Physiol 130:1443–1453
Bohnert HJ, Jensen RG (1996) Strategies for engineering water stress tolerance in plants. Trends Biotechnol 14:89–97
Das-Chatterjee A, Goswami L, Maitra S, Dastidar KG, Ray S, Majumder AL (2006) Introgression of a novel salt-tolerant L-myo-inositol 1-phosphate synthase from Porteresia coarctata (Roxb.) Tateoka (PcINO1) confers salt tolerance to evolutionary diverse organisms. FEBS Lett 580:3980–3988
Demmig-Adams B, Adams WW (1992) Photoprotection and other responses of plants to high light stress. Annual Rev Plant Physiol and Plant Mol Biol 43:599–626
Galinski EA (1993) Compatible solutes of halophilic eubacteria: molecular principles, water-solute interaction, stress protection. Experientia 49:487–496
Holmstrom K, Mantyla E, Welin B, Mandal A, Palva ET (1996) Drought tolerance in tobacco. Nature 379:683–684
Horsch RB, Fry JE, Hoffman NL, Eicholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
Kishore PBK, Hong Z, Miao G, Hu CA, Verma DPS (1995) Overexpression of [delta] pyrroline-5-carboxylase synthase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394
Kosugi S, Ohashi Y, Nakajima K, Arai Y (1990) An improved assay for β-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous β-glucuronidase activity. Plant Sci 70:133–140
Loewus FA, Dickinson MW (1982) Cyclitols. In: Loewus FA, Tanner W (eds) Encyclopedia of plant physiology, new series, vol. 13A, plant carbohydrates. Springer, Berlin, pp 94–216
Maguire TL, Collins GG, Sedgley M (1994) A modified CTAB DNA extraction procedure for plants belonging to the family Proteaceae. Plant Mol Biol Report 12:106–109
Majee M, Maitra S, Dastidar KG, Pattnaik S, Chatterjee A, Hait NC, Das KP, Majumder AL (2004) A novel salt-tolerant L-myo-inositol-1-phosphate synthase from Porteresia coarctata (Roxb.) Tateoka, a halophytic wild rice: molecular cloning, bacterial overexpression, characterization, and functional introgression into tobacco-conferring salt tolerance phenotype. J Biol Chem 279:28539–28552
Majumder AL, Johnson MD, Henry SA (1997) L-myo-Inositol-1-phosphate synthase. Biochim Biophys Acta 1348:245–256
Nishiguchi R, Takanami M, Oka A (1987) Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA4b. Mol Gen Genet 206:1–8
Nomura M, Ishitani M, Takabe T, Rai AK, Takabe T (1995) Synechococcus sp. PCC7942 transformed with Escherichia coli bet genes produces glycine betaine from choline and acquires resistance to salt stress. Plant Physiol 107:703–708
Peterbauer T, Puschenreiter M, Richter A (1998) Metabolism of galactosyl ononitol in seeds of Vigna umbellata. Plant Cell Physiol 39:334–341
Pilon-Smits EAH, Ebaskamp MJM, Paul MJ, Jeuken MJW, Weisbeek PJ, Smeekens SCM (1995) Improved performance of transgenic Fructan-accumulating tobacco under drought stress. Plant Physiol 107:125–130
Rhodes D, Samaras Y (1994) Genetic control of osmoregulation in plants. In: Strange K (ed) Cellular and molecular physiology of cell volume regulation. CRC, Boca Raton, pp 347–361
Sengupta S, Patra B, Ray S, Majumder AL (2008) Inositol methyl transferase from a halophytic wild rice Porteresia coarctata Roxb. (Tateoka): its expression pattern under abiotic stress. Plant Cell Environ 31:1442–1459
Shen B, Hohmann S, Jensen RG, Bohnert HJ (1999) Roles of sugar alcohols in osmotic stress adaptation replacement of glycerol by mannitol and sorbitol in yeast. Plant Physiol 121:45–52
Sheveleva E, Chmara W, Bohnert HJ, Jensen J (1997) Increased salt and drought tolerance by D-Ononitol production in transgenic Nicotiana tabacum L. Plant Physiol 115:1211–1219
Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a in plants and cyanobacteria. Photochem Photobiol 61:32–34
Tarczynski MC, Jensen RG, Bohnert HJ (1993) Stress protection in transgenic tobacco by production of the osmolyte mannitol. Science 259:508–510
Vernon DM, Bohnert HJ (1992) A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum crystallinum. EMBO J 11:2077–2085
Acknowledgments
The authors are indebted to Prof. Hans J. Bohnert for the research gift of McIMT1 clone. The work has supported by research grants (to ALM) from the Department of Biotechnology, Government of India.
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Patra, B., Ray, S., Richter, A. et al. Enhanced salt tolerance of transgenic tobacco plants by co-expression of PcINO1 and McIMT1 is accompanied by increased level of myo-inositol and methylated inositol. Protoplasma 245, 143–152 (2010). https://doi.org/10.1007/s00709-010-0163-3
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DOI: https://doi.org/10.1007/s00709-010-0163-3