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Structure analysis of sulfated polysaccharides extracted from green seaweed Ulva lactuca: experimental and density functional theory studies

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Abstract

The structure of sulfated polysaccharides extracted from green seaweed Ulva lactuca has been determined using NMR experiments. The obtained results indicate that this natural product is mainly composed of disaccharide A3S: [→4)-β-d-GlcA-(1 → 4)-α-l-Rha3S-(1→] and B3S: [→4)-α-l-IdoA-(1 → 4)-α-l-Rha3S-(1→]. The computational calculations using density functional theory (DFT) at the B3LYP/6–311+G(2d,p)//B3LYP/6–311G(d,p) model chemistries were also investigated to analyze the obtained structures based on the comparison of 1H and 13C NMR chemical shifts. The DFT results also confirm that the A3S structure is more dominating than the B3S one with the mean absolute error of 1H and 13C NMR chemical shifts, respectively, equal to 0.46 and 4.98 ppm for the A3S and to 2.07 and 5.03 ppm for the B3S.

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Acknowledgements

This research is funded by Vietnam Academy of Science and Technology (Project number VAST03.04/15-16).

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Authors and Affiliations

  1. Nhatrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong, Nha Trang, Viet Nam

    Thi Thanh Van Tran, Hai Bang Truong & Minh Ly Bui

  2. Institute of Research and Development, Duy Tan University, 03 Quang Trung, Da Nang, Viet Nam

    Bui The Huy & Duy Quang Dao

  3. Department of Chemistry, Changwon National University, Changwon, 51140, Korea

    Bui The Huy

  4. Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam

    Thi Thu Thuy Thanh

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  1. Thi Thanh Van Tran
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  2. Bui The Huy
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Correspondence to Duy Quang Dao.

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Tran, T.T.V., Huy, B.T., Truong, H.B. et al. Structure analysis of sulfated polysaccharides extracted from green seaweed Ulva lactuca: experimental and density functional theory studies. Monatsh Chem 149, 197–205 (2018). https://doi.org/10.1007/s00706-017-2056-z

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  • DOI: https://doi.org/10.1007/s00706-017-2056-z

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