Skip to main content
SpringerLink
Log in

Molecular characterization of a novel alternavirus infecting the entomopathogenic fungus Cordyceps chanhua

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

In this study, a novel double-stranded (ds) RNA mycovirus, named Cordyceps chanhua alternavirus 1 (CcAV1), was detected in the entomogenous fungus Cordyceps chanhua in China and characterized. The complete genome of CcAV1 is composed of three dsRNA segments: dsRNA 1 (3,512 bp), dsRNA 2 (2,655 bp), and dsRNA 3 (2,415 bp). Each of the three dsRNAs possesses a single open reading frame (ORF). dsRNA 1 encodes a putative RNA-dependent RNA polymerase (RdRp), and dsRNA 2 and dsRNA 3 encode hypothetical protein 1 (HP 1) and hypothetical protein 2 (HP 2), respectively. The predicted amino acid sequences of the putative RdRp, HP 1, and HP 2 had the highest identity of 66.99%, 49.30%, and 56.91%, respectively, to those of Aspergillus foetidus dsRNA mycovirus. A maximum-likelihood phylogenetic tree based on RdRp amino acid sequences showed that CcAV1 clustered with members of the proposed family “Alternaviridae”. Hence, we propose that Cordyceps chanhua alternavirus 1 is a novel member of the proposed family “Alternaviridae”.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Wang P, Yang G, Shi N, Huang B (2021) A novel gammapartitivirus from the entomopathogenic fungus Metarhizium brunneum. Arch Virol 166:977–981

    Article  CAS  Google Scholar 

  2. Kozlakidis Z, Herrero N, Ozkan S, Kanhayuwa L, Jamal A, Bhatti MF, Coutts RH (2013) Sequence determination of a quadripartite dsRNA virus isolated from Aspergillus foetidus. Arch Virol 158:267–272

    Article  CAS  Google Scholar 

  3. He H, Chen X, Li P, Qiu D, Guo L (2018) Complete genome sequence of a Fusarium graminearum double-stranded RNA virus in a newly proposed Family, Alternaviridae. Genome Announc 6:e00064-e118

    Article  Google Scholar 

  4. Osaki H, Sasaki A, Nomiyama K, Tomioka K (2016) Multiple virus infection in a single strain of Fusarium poae shown by deep sequencing. Virus Genes 52:835–847

    Article  CAS  Google Scholar 

  5. Hammond TM, Andrewski MD, Roossinck MJ, Keller NP (2008) Aspergillus mycoviruses are targets and suppressors of RNA silencing. Eukaryot Cell 7:350–357

    Article  CAS  Google Scholar 

  6. Wu CF, Aoki N, Takeshita N, Fukuhara T, Chiura HX, Arie T, Kotta-Loizou I, Okada R, Komatsu K, Moriyama H (2021) Unique terminal regions and specific deletions of the segmented double-stranded RNA genome of Alternaria alternata Virus 1, in the proposed Family Alternaviridae. Front Microbiol 12:773062

    Article  Google Scholar 

  7. Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140:179–187

    Article  CAS  Google Scholar 

  8. Zhang X, Xie Y, Zhang F, Sun H, Zhai Y, Zhang S, Yuan H, Zhou L, Gao F, Li H (2019) Complete genome sequence of an alternavirus from the phytopathogenic fungus Fusarium incarnatum. Arch Virol 164:923–925

    Article  CAS  Google Scholar 

  9. Li Z, Luan F, Hywel J, Zhang S, Chen M, Huang B, Sun C, Chen Z, Li C, Tan Y, Dong J (2021) Biodiversity of cordycipitoid fungi associated with Isaria cicadae Miquel II: Teleomorph discovery and nomenclature of chanhua, an important medicinal fungus in China. Mycosystema 40(1):95–107

    Google Scholar 

  10. Taylor JW (2011) One Fungus = one name: DNA and fungal nomenclature twenty years after PCR. IMA Fungus 2:113–120

    Article  Google Scholar 

  11. Shi N, Yang G, Wang P, Wang Y, Yu D, Huang B (2019) Complete genome sequence of a novel partitivirus from the entomogenous fungus Beauveria bassiana in China. Arch Virol 164:3141–3144

    Article  CAS  Google Scholar 

  12. Herrero N, Dueñas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microb 78:8523–8530

    Article  CAS  Google Scholar 

  13. Coutts RHA, Livieratos IC (2003) A rapid method for sequencing the 5′- and 3′- termini of double-stranded RNA viral templates using RLM-RACE. J Phytopathol 151:525–527

    Article  CAS  Google Scholar 

  14. Wen C, Wan X, Zhang Y et al (2021) Molecular characterization of the first alternavirus identified in Fusarium oxysporum. Viruses 13(10):2026

    Article  CAS  Google Scholar 

  15. Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20:1160–1166

    Article  CAS  Google Scholar 

  16. Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    Article  CAS  Google Scholar 

  17. Gilbert KB, Holcomb EE, Allscheid RL, Carrington JC (2019) Hiding in plain sight: new virus genomes discovered via a systematic analysis of fungal public transcriptomes. PLoS ONE 14(7):e02192

    Article  Google Scholar 

Download references

Funding

This work was supported by the National Natural Science Foundation of China (Grant no. 32172473) and the Anhui Natural Science Foundation (Grant no. 1908085MC56).

Author information

Author notes

  1. Yuxiang Zhang and Najie Shi contributed equally to this study and share first authorship.

Authors and Affiliations

  1. Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China

    Yuxiang Zhang, Najie Shi, Ping Wang, Qiuyan Zhu & Bo Huang

  2. School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China

    Guogen Yang

Authors
  1. Yuxiang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Najie Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiuyan Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guogen Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bo Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bo Huang.

Ethics declarations

Conflict of interest

The authors have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Ioly Kotta-Loizou.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Y., Shi, N., Wang, P. et al. Molecular characterization of a novel alternavirus infecting the entomopathogenic fungus Cordyceps chanhua. Arch Virol 167, 1467–1470 (2022). https://doi.org/10.1007/s00705-022-05446-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-022-05446-2

Search

Navigation