Abstract
A novel double-stranded RNA (dsRNA) mycovirus, named “Fusarium incarnatum alternavirus 1” (FiAV1), was found in Fusarium incarnatum strain LY003-07, the causal agent of peony root rot. The complete genome of FiAV1 is composed of three dsRNA segments, dsRNA1 (3548 nt), dsRNA2 (2514 nt), and dsRNA3 (2498 nt), with one open reading frame on each of their positive-sense strands. As found in other viruses of the proposed family Alternaviridae, the positive-sense strand of each genomic dsRNA of FiAV1 has a poly(A) tail and the 5’-terminal nonamer sequence 5’-GGCTGTGTG-3’. Based on a multiple sequence alignment and phylogenetic analysis based on the RdRps amino acid sequence, FiAV1 is suggested to be a new strain of a potential new species, for which the name “Fusarium alternavirus 1” is proposed, that includes Fusarium poae alternavirus 1 (FpAV1) and Fusarium graminearum alternavirus 1 (FgAV1/AH11) of the proposed family “Alternaviridae”. This is the first report of a mycovirus of the proposed family “Alternaviridae” that infects F. incarnatum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kozlakidis Z, Herrero N, Ozkan S, Kanhayuwa L, Jamal A, Bhatti MF, Coutts RHA (2013) Sequence determination of a quadripartite dsRNA virus isolated from Aspergillus foetidus. Arch Virol 158:267–272
Kotta-Loizou I, Coutts RHA (2017) Mycoviruses in Aspergilli: a comprehensive review. Front Microbiol 8:1699
Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140:179–187
Hammond TM, Andrewski MD, Roossinck MJ, Keller NP (2008) Aspergillus mycoviruses are targets and suppressors of RNA silencing. Eukaryot Cell 7:350–357
Osaki H, Sasaki A, Nomiyama K, Tomioka K (2016) Multiple virus infection in a single strain of Fusarium poae shown by deep sequencing. Virus Genes 52:835–847
He H, Chen X, Li P, Qiu D, Guo L (2018) Complete genome sequence of a Fusarium graminearum double-stranded RNA virus in a newly proposed family, Alternaviridae. Genome Announc 6:e00064-18
Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus infected plant and fungal tissue. Phytopathology 69(8):854–858
Xie J, Wei D, Jiang D, Fu Y, Li G, Ghabrial S, Peng Y (2006) Characterization of debilitation associated mycovirus infecting the plant-pathogenic fungus Sclerotinia sclerotiorum. J Gen Virol 87:241–249
Chiba S, Salaipeth L, Lin YH, Sasaki A, Kanematsu S, Suzuki N (2009) A novel bipartite double-stranded RNA mycovirus from the white root rot fungus Rosellinia necatrix: molecular and biological characterization, taxonomic considerations, and potential for biological control. J Virol 83:12801–12812
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Acknowledgements
This research was financially supported by the National Special Fund for Agro-Scientific Research in the Public Interest of China (201503112) and Henan Province Agricultural Science and Technology Research Project (182102110004). The authors would like to thank Prof. Jiatao Xie for advice during the preparation of the manuscript.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
All authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Handling Editor: Robert H. A. Coutts.
Data Deposition Information are available at http://www.ncbi.nlm.nih.gov/.
Electronic supplementary material
Below is the link to the electronic supplementary material.
705_2018_4128_MOESM1_ESM.docx
Supplementary material 1 (DOCX 311 kb) Supplemental Figure 1 Comparison of the 5’- and 3’- termini of three genomic dsRNA segments of FiAV1
705_2018_4128_MOESM2_ESM.pdf
Supplementary material 2 (PDF 480 kb) Supplemental Figure 2 Phylogenetic analysis of FiAV1 and selected members of the families Alternaviridae (▲, FiAV1 in red▲), Fusariviridae (⃞), Hypoviridae (⃝) and Totiviridae based on the aligned RdRp aa sequences of the viruses by the neighbor-joining method using the program MEGA 7.0. Bootstrap values (1000 replicates) are shown at the nodes. The scale bar (0.1) corresponds to genetic distance
Rights and permissions
About this article
Cite this article
Zhang, X., Xie, Y., Zhang, F. et al. Complete genome sequence of an alternavirus from the phytopathogenic fungus Fusarium incarnatum. Arch Virol 164, 923–925 (2019). https://doi.org/10.1007/s00705-018-04128-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-018-04128-2