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Evidence of coinfection of pigs with African swine fever virus and porcine circovirus 2

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Abstract

Archival swine DNA samples from Indonesia and Mongolia, some of which were previously shown to be positive for African swine fever virus, were screened for the presence of porcine circovirus 2 (PCV-2) and porcine circovirus 3 (PCV-3) by PCR. Samples from both countries were positive for PCV-2 (three from Mongolia and two from Indonesia), while none were positive for PCV-3. The PCV-2 amplicons were sequenced, and phylogenetic analysis revealed that the PCV-2 strains belonged to four different genotypes: PCV-2a (Mongolia), PCV-2b (Mongolia and Indonesia), PCV-2d (Indonesia), and PCV-2g (Mongolia). This is the first report of ASFV/PCV-2 coinfection in pigs and the first report of the presence of PCV-2 in Mongolia.

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Fig. 1

Data availability statement

The data that support the findings of this study are openly available in GenBank at https://www.ncbi.nih.gov/genbank/, reference numbers MZ422380 to MZ422384.

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Acknowledgements

This study was supported by funds from the IAEA Peaceful Uses Initiative (PUI) VETLAB Network. The sequences were generated through the Sequencing Services of the Animal Production and Health sub-programme of the Joint Food and Agricultural Organization of the United Nations/International Atomic Energy Agency (IAEA) Division.

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Authors and Affiliations

  1. Animal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Centre, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, PO Box 100, 1400, Vienna, Austria

    William G. Dundon, Tirumala B. K. Settypalli, Giovanni Cattoli & Charles E. Lamien

  2. Department of Animal Medicine, Production and Health, University of Padova, Viale dell’Università 16, 35020, Legnaro, Italy

    Giovanni Franzo

  3. Indonesian Research Center for Veterinary Science (IRCVS), Bogor, Indonesia

    N.L.P. Indi Dharmayanti, Indrawati Sendow & Atik Ratnawati

  4. State Veterinary Laboratory, Ulaanbatar, Mongolia

    Ulaankhuu Ankhanbaatar & Tserenchimed Sainnokhoi

  5. Faculty of Agriculture and Natural Resources, School of Veterinary Medicine, University of Namibia, Neudamm Campus, Private Bag 13301, Windhoek, Namibia

    Umberto Molini

  6. Animal Production and Health Laboratory, IAEA, Freidenstrasse, 1, 2444, Seibersdorf, Austria

    William G. Dundon

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  1. William G. Dundon
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Correspondence to William G. Dundon.

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W.G. Dundon is on the Editorial Board of Archives of Virology.

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No animal work was performed for this study.

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Dundon, W.G., Franzo, G., Settypalli, T.B.K. et al. Evidence of coinfection of pigs with African swine fever virus and porcine circovirus 2. Arch Virol 167, 207–211 (2022). https://doi.org/10.1007/s00705-021-05312-7

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  • DOI: https://doi.org/10.1007/s00705-021-05312-7

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