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Codiaeum variegatum in Pakistan harbours pedilanthus leaf curl virus and papaya leaf curl virus as well as a newly identified betasatellite

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Abstract

Codiaeum variegatum (common name, garden croton) is an ornamental plant grown for its bright yellow variegated leaf morphology. Two C. variegatum plants with upward leaf curling and vein swelling symptoms were collected in Faisalabad, Pakistan. Sequencing of clones obtained by PCR amplification with specific primers showed one plant infected with the monopartite begomoviruses pedilanthus leaf curl virus (PeLCV) and papaya leaf curl virus (PaLCuV) and the other to be infected with only PeLCV. Both plants also harboured a betasatellite that was distinct from all previously identified betasatellites, for which the name “codiaeum leaf curl betasatellite” (CoLCuB) is proposed. This is the first identification of a begomovirus and an associated betasatellite infecting C. variegatum in Pakistan. Both PeLCV and PaLCuV cause problems in a number of crop plants, and C. variegatum may act as a reservoir for these agriculturally important viruses. The precise impact and geographical distribution of the newly identified CoLCuB will be investigated.

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Acknowledgements

The authors are grateful to Dr. Imran Amin, Dr. Brian E. Scheffler, and Ms. Fanny Liu for assistance with sequencing. This material is based upon work supported by the “Pak-US Cotton Productivity Enhancement Program” of the International Centre for Agricultural Research in the Dry Areas (ICARDA), funded by the United States Department of Agriculture (USDA), Agricultural Research Service (ARS), under agreement no. 58-6402-0-178F. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the USDA or ICARDA. RWB was supported by the Higher Education Commission, Government of Pakistan, under the ‘Foreign Faculty Hiring Program’.

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Author notes

  1. Ghulam Rasool

    Present address: Department of Plant Breeding and Genetics, Faculty of Agriculture Sciences (FAS), Ghazi University, D. G. Khan, Pakistan

Authors and Affiliations

  1. Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan

    Afzal Akram, Aqsa Hafeez Khan, Ghulam Rasool, Shahid Mansoor, Rob W. Briddon & Muhammad Saeed

  2. Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan

    Afzal Akram, Aqsa Hafeez Khan & Ghulam Rasool

  3. Département de Biologie, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada

    Afzal Akram & Peter Moffett

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  1. Afzal Akram
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Correspondence to Muhammad Saeed.

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Akram, A., Khan, A.H., Rasool, G. et al. Codiaeum variegatum in Pakistan harbours pedilanthus leaf curl virus and papaya leaf curl virus as well as a newly identified betasatellite. Arch Virol 165, 1877–1881 (2020). https://doi.org/10.1007/s00705-020-04633-3

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