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Establishment and application of an indirect ELISA for porcine circovirus 3

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Abstract

Porcine circovirus type 3 (PCV3) was initially reported in 2016 in the United States of America. Since then, the virus has been detected on swine farms in Poland, South Korea, and China using PCR. However, a serological survey of PCV3 in pig populations has never been conducted. In this study, for the first time, we established an indirect enzyme-linked immunosorbent (ELISA) assay and performed a national retrospective serological survey for PCV3. Our results showed that the PCV3-postive rate increased from 22.35% to 51.88% between 2015 and 2017. The above results suggest PCV3 has spread widely in China with increased positive rates since 2015.

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Author notes

  1. Junhua Deng and Xiangdong Li contributed equally to this study.

Authors and Affiliations

  1. National Research Center for Veterinary Medicine, High-Tech District, Luoyang, China

    Junhua Deng, Xiangdong Li, DingDing Zheng, Yanwei Wang, Lingyan Chen, Huanhuan Song, Tongyan Wang, Yuxin Huang, Wenqiang Pang & Kegong Tian

  2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China

    Kegong Tian

Authors
  1. Junhua Deng
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  2. Xiangdong Li
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  3. DingDing Zheng
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  4. Yanwei Wang
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  5. Lingyan Chen
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  6. Huanhuan Song
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  7. Tongyan Wang
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  8. Yuxin Huang
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  9. Wenqiang Pang
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  10. Kegong Tian
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Corresponding author

Correspondence to Kegong Tian.

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Funding

This study was funded by the Major Science and Technology Projects in Henan Province with Grant No.171100110200 and Luoyang Heluo Talent Plan (Dr. Kegong Tian).

Conflict of interest

The authors declare that they do not have any conflict of interest.

Ethical approval

All animal trials were approved by the Animal Care and Ethics Committee of China National Research Center for Veterinary Medicine.

Additional information

Handling Editor: Zhenhai Chen.

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Deng, J., Li, X., Zheng, D. et al. Establishment and application of an indirect ELISA for porcine circovirus 3. Arch Virol 163, 479–482 (2018). https://doi.org/10.1007/s00705-017-3607-7

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  • DOI: https://doi.org/10.1007/s00705-017-3607-7

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