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Complete genome sequence of rice virus A, a new member of the family Tombusviridae

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Abstract

An evaluation of the virus population in rice plants using next-generation sequencing technologies resulted in the discovery of a new RNA virus, tentatively named rice virus A (RVA). The complete RVA genome sequence was determined and analyzed, revealing a genome organization resembling that of viruses classified in the genera Aureusvirus, Tombusvirus and Zeavirus within the family Tombusviridae. With 4,832 nucleotides, the RVA genome may be the largest monopartite genome sequenced to date in the family Tombusviridae. The 453-amino acid RVA coat protein shares the highest identity with the gp3 protein of an unclassified carascovirus, SF1 (GenBank accession no. KF510027) isolated from San Francisco wastewater, rather than the coat protein of any known member of the family Tombusviridae. These novel characteristics represent a significant divergence from the genomes of viruses belonging to the sixteen existing genera of the family Tombusviridae, demonstrating that RVA is likely a new family member.

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Acknowledgements

This work was supported by the KRIBB initiative program funded by korean government (MISP: Ministry of Science, ICT and Future Planning).

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Author notes

  1. Fumei Zhao

    Present address: Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

Authors and Affiliations

  1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305–806, South Korea

    Fumei Zhao, Dasom Baek, Davaajargal Igori & Jae Sun Moon

  2. Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, South Korea

    Dasom Baek, Davaajargal Igori & Jae Sun Moon

  3. Crop Foundation Division, National Institute of Crop Science, RDA, Wanju, 55365, South Korea

    Ju Young Bae, Sang-Min Kim, Ji-Eun Ra, Ki Do Park & Bong Choon Lee

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  1. Fumei Zhao
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  2. Dasom Baek
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  3. Davaajargal Igori
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Corresponding authors

Correspondence to Bong Choon Lee or Jae Sun Moon.

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This study was founded by the KRIBB initiative program funded by korean government (MISP: Ministry of Science, ICT and Future Planning).

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Zhao, F., Baek, D., Igori, D. et al. Complete genome sequence of rice virus A, a new member of the family Tombusviridae . Arch Virol 162, 3247–3250 (2017). https://doi.org/10.1007/s00705-017-3472-4

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  • DOI: https://doi.org/10.1007/s00705-017-3472-4

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