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Complete genome characterization of cacao leafroll virus, a newly described cacao-infecting polerovirus

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Abstract

The complete genome sequence of cacao leafroll virus (CaLRV; family Solemoviridae, genus Polerovirus) was determined by high-throughput sequencing of total RNA isolated from symptomatic cacao Theobroma cacao L. plants (n = 4). The CaLRV genome sequences ranged from 5,976 to 5,997 nucleotides (nt) in length and contained seven open reading frames (ORFs). Nucleotide and amino acid (aa) sequence comparisons showed that, among selected well-characterized poleroviruses, the CaLRV genome shared the highest nt sequence identity of 62% with that of potato leafroll virus (PLRV, NC_076505). A comparison of the predicted aa sequence of the CaLRV coat protein indicated that cotton leafroll dwarf virus (CLRDV, NC_014545) and melon aphid-borne yellows virus (MABYV, NC_010809) were the closest relatives, sharing 57% aa sequence identity. Bayesian phylogenetic analysis based on complete genome sequences showed that CaLRV grouped with well-characterized poleroviruses that cause diseases of cereal and vegetable crops. During the course of publishing this work, the nearly complete genome sequence of a member of the same polerovirus species, referred to as “cacao polerovirus” (OR605721), with which CaLRV shares 99% nt sequence identity, was reported.

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Data availability

Nucleotide sequences of the newly reported CaLRV isolates are available in the NCBI GenBank database and have been assigned the accession number, OR423047-50.

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Funding

This study was supported by a USDA-ARS Non-Assistance Cooperative Agreement to JKB: Number: 6038-21000-025-001-S titled: Discovery Sequencing for Improved PCR/qPCR Diagnostic Test Development to Identify the Presence of Badnaviruses in Cacao Germplasm Collections.

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Authors and Affiliations

  1. School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA

    Raphael O. Adegbola, Cory V. Keith & Judith K. Brown

  2. USDA-ARS Subtropical Horticultural Research Station, Miami, FL, 33158, USA

    Osman Gutierrez

  3. USDA-ARS Tropical Agriculture Research Station, Mayaguez, PR, 00680, USA

    Ricardo Goenaga

Authors
  1. Raphael O. Adegbola
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  2. Cory V. Keith
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  4. Ricardo Goenaga
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Contributions

All authors contributed to the conception and design of the study. Funding was procured by Judith K. Brown. Laboratory experiments, data collection, and analysis were performed by Raphael O. Adegbola and Cory V. Keith. The first draft of the manuscript was written by Raphael O. Adegbola. All authors read, edited, and approved the final manuscript.

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Correspondence to Judith K. Brown.

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Communicated by Elvira Fiallo-Olivé

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Adegbola, R.O., Keith, C.V., Gutierrez, O. et al. Complete genome characterization of cacao leafroll virus, a newly described cacao-infecting polerovirus. Arch Virol 169, 83 (2024). https://doi.org/10.1007/s00705-024-06013-7

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