Skip to main content
SpringerLink
Log in

The complete genomic sequence of a novel alphapartitivirus from Bipolaris maydis, the causal agent of corn southern leaf blight

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A novel double-stranded RNA (dsRNA) mycovirus, designated Bipolaris maydis partitivirus 1 (BmPV1), was isolated from the plant pathogenic fungus Bipolaris maydis. The BmPV1 genome has two dsRNA segments. The larger segment (1,930 bp) has a single open reading frame (ORF) with a conserved RNA-dependent RNA polymerase domain. The smaller segment (1,790 bp) contains a single ORF encoding a putative coat protein. Homology searches and phylogenetic analysis indicated that BmPV1 is representative of a new species within the genus Alphapartitivirus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Blawid R, Stephan D, Maiss E (2008) Alphacryptovirus and Betacryptovirus. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of Virology. Academic Press, New York, pp 98–104

    Chapter  Google Scholar 

  2. Ghabrial SA, Caston JR, Jiang DH, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368

    Article  PubMed  Google Scholar 

  3. Ghabrial SA, Suzuki N (2009) Viruses of Plant Pathogenic Fungi. Annu Rev Phytopathol 47:353–384

    Article  CAS  PubMed  Google Scholar 

  4. Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Dodds JA (1979) Isolation and analysis of doublestranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  Google Scholar 

  6. Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Suzuki N, Jiang D (2014) Taxonomic reorganization of family Partitiviridae, and other recent progress in partitivirus research. Virus Res 188:128–141

    Article  CAS  PubMed  Google Scholar 

  7. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3(8):632–642

    Article  CAS  PubMed  Google Scholar 

  8. Ochoa WF, Havens WM, Sinkovits RS, Nibert ML, Ghabrial SA, Baker TS (2008) Partitivirus structure reveals a 120-subunit, helix-rich capsid with distinctive surface arches formed by quasisymmetric coat-protein dimers. Structure 16:776–786

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Pan JH, Dong LP, Lin L, Ochoa WF, Sinkovits RS, Havens WM, Nibert ML, Baker TS, Ghabrial SA, Tao YZJ (2009) Atomic structure reveals the unique capsid organization of a dsRNA virus. PNAS 106:4225–4230

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90(Pt 6):1423–1432

    Article  CAS  PubMed  Google Scholar 

  11. Tang JH, Ochoa WF, Li H, Havens WM, Nibert ML, Ghabrial SA, Baker TS (2010) Structure of Fusarium poae virus 1 shows conserved and variable elements of partitivirus capsids and evolutionary relationships to picobirnavirus. J Struct Biol 172:363–371

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Tang JH, Pan JH, Havens WM, Ochoa WF, Guu TSY, Ghabrial SA, Nibert ML, Tao YZJ, Baker TS (2010) Backbone trace of partitivirus capsid protein from electron cryomicroscopy and homology modeling. Biophys J 99:685–694

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Xie JT, Jiang DH (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. 31201474 and No. 31301638), the National Key Technology Research and Development Program (2014BAD23B00) and the Opening Fund of Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province.

Author information

Author notes

  1. Qingchao Deng and Haoran Wang contributed equally to this work.

Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Grain Industry, Engineering Research Center of Ecology and Agricultural Use of Wetland, China Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China

    Qingchao Deng, Haoran Wang, Cong Li, Pei Li, Shouguo Fang & Songbai Zhang

  2. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, Guizhou, China

    Song Yang & Zhuo Chen

  3. State Key Laboratory Breeding Base for Sustainable Control of Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Fei Yan & Songbai Zhang

Authors
  1. Qingchao Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haoran Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shouguo Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Song Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fei Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Songbai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zhuo Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Songbai Zhang or Zhuo Chen.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent: Informed consent was obtained from all individual participants included in the study. All authors have seen the manuscript and approved to submit to “Archives of Virology”.

Electronic supplementary material

Below is the link to the electronic supplementary material.

705_2017_3356_MOESM1_ESM.tif

Supplementary Fig. 3 dsRNAs electrophoresis pattern of B. maydis strain JZ01~JZ14. Lane M, DL15,000 DNA Marker (Takara); Lane 1~14, JZ01~14. (TIFF 497 kb)

Supplementary material 2 (TXT 3 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Deng, Q., Wang, H., Li, C. et al. The complete genomic sequence of a novel alphapartitivirus from Bipolaris maydis, the causal agent of corn southern leaf blight. Arch Virol 162, 2433–2436 (2017). https://doi.org/10.1007/s00705-017-3356-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-017-3356-7

Keywords

Search

Navigation