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Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014

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Abstract

Members of avian leukosis virus subgroup J (ALV-J) cause various diseases associated with tumor formation and decreased fertility, resulting in major economic losses in the poultry industry worldwide. To assess the status of ALV-J infection in meat-type chickens in southern China, the molecular epidemiology of ALV-J strains was investigated. A total of 265 clinical samples collected from southern China from 2013 to 2014 were investigated in this study for the presence of ALV-J, which resulted in 12 virus isolates. Phylogenetic analysis showed that 91.7 % (11/12) of the ALV-J isolates have possessed high homology to Chinese layer isolates and belong to one subgroup. One of the ALV isolates (designated GD1411-1) was relatively closely related to the ALV-J broiler isolates, indicating that the GD1411-1 isolate might be a transition strain. Several unique nucleotide substitutions in gp85 and the U3 region were detected in all 12 ALV-J isolates. This study provides some interesting information on the molecular characterization of ALV-J isolates. These findings will be beneficial for understanding of the pathogenic mechanism of ALV-J infection.

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Acknowledgments

This study was supported by the Natural Science Foundation of Guangdong Province (Grant No. S2013030013313), National Natural Science Foundation of China (Grant No. 31472217) and Guangdong Province Science and Technology Plan Project (Grant Nos. 2012B020306002, 2012B091100078).

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    Authors and Affiliations

    1. Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Key Laboratory of Chicken Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Ministry of Agriculture, Guangzhou, 510642, People’s Republic of China

      Wencheng Lin, Xinjian Li, Zhenkai Dai, Xinheng Zhang, Feng Chen & Qingmei Xie

    2. College of Veterinary Medicine, Shandong Agricultural University, Taian, 271018, People’s Republic of China

      Shuang Chang & Peng Zhao

    3. USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823, USA

      Huanmin Zhang

    4. Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, 510642, Guangdong, People’s Republic of China

      Wencheng Lin, Feng Chen & Qingmei Xie

    5. South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou, 510642, People’s Republic of China

      Wencheng Lin & Qingmei Xie

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    1. Wencheng Lin
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    2. Xinjian Li
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    3. Zhenkai Dai
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    4. Xinheng Zhang
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    7. Huanmin Zhang
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    9. Qingmei Xie
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    Correspondence to Qingmei Xie.

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    W. Lin and X. Li contributed equally to this work.

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    Lin, W., Li, X., Dai, Z. et al. Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014. Arch Virol 161, 3039–3046 (2016). https://doi.org/10.1007/s00705-016-3003-8

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