Abstract
Hemagglutinin neuraminidase (HN) is a membrane protein of Newcastle disease virus (NDV) with the ability to induce apoptosis in many transformed cell lines. TNF-α is a multi-factorial protein that regulates cell survival, differentiation and apoptosis. In a previous study, we reported that HN protein induces apoptosis by downregulating NF-κB expression. Further, we speculated that downregulation of NF-κB expression might sensitize HeLa cells to TNF-α-mediated apoptosis. Therefore, the present study was undertaken to investigate if HN protein could sensitize HeLa cells to TNF-α and to examine the apoptotic potential of the HN protein and TNF-α in combination. The results revealed that the pro-apoptotic effects were more pronounced with the combination of HN and TNF-α than with HN or TNF-α alone, which indicates that the HN protein indeed sensitized the HeLa cells to TNF-α-induced cell death. The results of the study provide a mechanistic insight into the apoptotic action of HN protein along with TNF-α, which could be valuable in treating tumor types that are naturally resistant to TNF-α.
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We are thankful to the director, Indian Veterinary Research Institute, Izatnagar, for providing necessary facilities.
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Department of Science and Technology (DST), Government (grant no. SR/SO/AS-0062/2007).
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All applicable national and institutional guidelines for the care and use of animals were followed.
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The authors declare no conflict of interest.
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Rajmani, R.S., Gupta, S.K., Singh, P.K. et al. HN protein of Newcastle disease virus sensitizes HeLa cells to TNF-α-induced apoptosis by downregulating NF-κB expression. Arch Virol 161, 2395–2405 (2016). https://doi.org/10.1007/s00705-016-2923-7
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DOI: https://doi.org/10.1007/s00705-016-2923-7