Abstract
Newcastle disease virus (NDV), an avian paramyxovirus, induces apoptosis in chicken embryo fibroblast (CEF) cells. In the present investigation, the ability of haemagglutinin-neuraminidase (HN) protein of NDV to cause apoptosis in CEF cells was examined. The results revealed that cells expressing the HN protein demonstrated decreased DNA content, phosphatidylserine exposure and increased cytoplasmic vacuolation. Up-regulation of caspase-1, -9, -8, -3, loss of mitochondrial transmembrane potential and an increase in oxidative stress were also observed in cells expressing the HN protein. Based on the above results it can be concluded that HN protein of NDV causes apoptosis in CEF cells.
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Acknowledgments
The first author wishes to thank University Grants Commission, Govt. of India, for providing financial support in the form of Junior Research Fellowship. He also thanks the Joint Director, Centre for Animal Disease Research and Diagnosis, and the Director, Indian Veterinary Research Institute, for providing necessary facilities to carry out this work.
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Ravindra, P.V., Tiwari, A.K., Sharma, B. et al. HN protein of Newcastle disease virus causes apoptosis in chicken embryo fibroblast cells. Arch Virol 153, 749–754 (2008). https://doi.org/10.1007/s00705-008-0057-2
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DOI: https://doi.org/10.1007/s00705-008-0057-2