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Lysosomes serve as a platform for hepatitis A virus particle maturation and nonlytic release

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Abstract

Early studies on hepatitis A virus (HAV) in cell culture demonstrated the inclusion of several viral particles in an intracellular lipid-bilayer membrane. However, the origin of these virus-associated membranes and the mechanism for the non-lytic release of HAV into bile are still unknown. Analyzing the association of this virus with cell organelles, we found that newly synthesized HAV particles accumulate in lysosomal organelles and that lysosomal enzymes are involved in the maturation cleavage of the virion. Furthermore, by inhibiting the processes of fusion of lysosomes with the plasma membrane, we found that the nonlytic release of HAV from infected cells occurs via lysosome-related organelles.

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Acknowledgments

We thank Mediagnost, Reutlingen, Germany, for providing the mAb anti-HAV IgG 7E7. This work was supported by grant BFK-no. 02/803/08 of the University of Bremen, Bremen, Germany, and by the Tönjes-Vagt-Stiftung, Project XXIX, Bremen, Germany.

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Authors and Affiliations

  1. Laboratory of Virus Research, University of Bremen, Leobener Straße/UFT, 28359, Bremen, Germany

    Nicole Seggewiß, Dajana Paulmann & Andreas Dotzauer

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  1. Nicole Seggewiß
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  2. Dajana Paulmann
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  3. Andreas Dotzauer
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Correspondence to Andreas Dotzauer.

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Seggewiß, N., Paulmann, D. & Dotzauer, A. Lysosomes serve as a platform for hepatitis A virus particle maturation and nonlytic release. Arch Virol 161, 43–52 (2016). https://doi.org/10.1007/s00705-015-2634-5

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  • DOI: https://doi.org/10.1007/s00705-015-2634-5

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