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P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts

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Abstract

The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.

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Acknowledgments

This work was funded by National Science and Technology Support Program (2012BAD19B03), the China 973 Program (2010CB126203), the Special Fund for Agro-Scientific Research in the Public Interest of China (201003031), 863 program (2007AA10Z414), the Zhejiang Provincial Science and Technology Project (2010C12027), and the Zhejiang Provincial Foundation for Natural Science (Z305165 and Y3090657). We thank Professor M. J. Adams, Stevenage, UK, for help in correcting the English of the manuscript.

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Authors and Affiliations

  1. College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, 321004, China

    Xiao-Ya Liu

  2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Plant Protection and Biotechnology, Ministry of Agriculture, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Xiao-Ya Liu, Jian Yang, Li Xie, Jing Li, Xi-Jiao Song, Jian-Ping Chen & Heng-Mu Zhang

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Correspondence to Jian-Ping Chen or Heng-Mu Zhang.

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Liu, XY., Yang, J., Xie, L. et al. P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts. Arch Virol 160, 1211–1217 (2015). https://doi.org/10.1007/s00705-015-2382-6

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  • DOI: https://doi.org/10.1007/s00705-015-2382-6

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