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The prevalence of duck hepatitis A virus types 1 and 3 on Korean duck farms

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Abstract

This study reports the prevalence of duck hepatitis A virus (DHAV) types 1 and 3 on Korean duck farms. By RT-nested PCR assays specific for DHAV-1 or DHAV-3, DHAV-1 was detected in 9 of 157 liver samples (5.7 %) from 2 of 30 farms (6.7 %), and DHAV-3 was positive in 104 of 157 liver samples (66.2 %) from 23 of 30 farms (76.7 %). Dual infections with DHAV-1 and DHAV-3 were detected in 23 of 157 samples (14.6 %) from 5 of 30 farms (16.7 %). The data indicate that DHAV-3 infections are prevalent and that DHAV-1 reemerged in Korea, resulting in dual infections on several farms. Our data will help to establish a vaccination policy against DHAV-1 and DHAV-3 in Korea.

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Acknowledgments

This study was supported by a grant from the Korea Basic Science Institute (C33730) and Chonnam National University (2012), Republic of Korea.

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Authors and Affiliations

  1. Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Mahmoud Soliman, Mia Madel Alfajaro, Min-Hee Lee, Young-Ju Jeong, Deok-Song Kim, Kyu-Yeol Son, Kyoung-Oh Cho & Mun-Il Kang

  2. Division of Life Science, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Joseph Kwon & Jong-Soon Choi

  3. Jeollanam-do Livestock Sanitation Office, Gangin, 527-822, Republic of Korea

    Jong-Soo Lim, Jong-Sung Choi & Tae-Uk Lee

Authors
  1. Mahmoud Soliman
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  2. Mia Madel Alfajaro
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  3. Min-Hee Lee
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  4. Young-Ju Jeong
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  5. Deok-Song Kim
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  6. Kyu-Yeol Son
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  7. Joseph Kwon
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  8. Jong-Soon Choi
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  9. Jong-Soo Lim
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  10. Jong-Sung Choi
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  11. Tae-Uk Lee
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  12. Kyoung-Oh Cho
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  13. Mun-Il Kang
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Corresponding authors

Correspondence to Kyoung-Oh Cho or Mun-Il Kang.

Additional information

M. Soliman and M. M. Alfajaro contributed equally to this paper.

Electronic supplementary material

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705_2014_2264_MOESM1_ESM.tif

Supplementary Fig. 1 Genomic structure of duck hepatitis A virus (DHAV). The figure depicts the location of each primer pair specific for the VP1 region of DHAV-1 or DHAV-3 used for detection and/or sequencing. (TIFF 966 kb)

705_2014_2264_MOESM2_ESM.tif

Supplementary Fig. 2 Phylogenetic trees based on the partial nucleotide (A) and deduced amino acid (B) sequences of the VP1 region of 32 Korean DHAV-1 isolates, indicating their genetic relationships to other known DHAV strains or isolates. Trees were generated using the neighbor-joining method with 1000 bootstrapping replicates in the MEGA 5.1 program. The bar indicates the genetic distance, and the black circle indicates the Korean isolates. Other known reference DHAV-2 and 3 strains listed in Supplementary Table 4 are replaced by triangles, in which the height of the triangle represents the number of sequences, and the width represents the genetic diversity inside the cluster. (TIFF 5531 kb)

705_2014_2264_MOESM3_ESM.tif

Supplementary Fig. 3 Phylogenetic trees based on the partial nucleotide (A) and deduced amino acid (B) sequences of the VP1 region of 83 Korean DHAV-3 isolates, indicating their genetic relationships to other known DHAV strains or isolates. Trees were generated using the neighbor-joining method with 1000 bootstrapping replicates in the MEGA 5.1 program. The bar indicates the genetic distance, and the black square indicates the Korean isolates. Other known reference DHAV-1 and 2 strains listed in Supplementary Table 5 are replaced by triangles, in which the height of the triangle represents the number of sequences, and the width represents the genetic diversity inside the cluster. (TIFF 13936 kb)

Supplementary material 4 (DOCX 31 kb)

Supplementary material 8 (DOCX 45 kb)

Supplementary material 7 (DOCX 35 kb)

Supplementary material 2 (DOCX 15 kb)

Supplementary material 3 (DOCX 16 kb)

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Soliman, M., Alfajaro, M.M., Lee, MH. et al. The prevalence of duck hepatitis A virus types 1 and 3 on Korean duck farms. Arch Virol 160, 493–498 (2015). https://doi.org/10.1007/s00705-014-2264-3

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  • DOI: https://doi.org/10.1007/s00705-014-2264-3

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