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Complete genomic sequence of the Vibrio alginolyticus lytic bacteriophage PVA1

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Abstract

A novel Vibrio alginolyticus lytic bacteriophage was isolated from sewage samples obtained from a local aquatic market. Morphological analysis revealed that the phage, designated as PVA1, belonged to the family Podoviridae. The complete genomic sequence of phage PVA1 contained 41,529 bp with a G + C content of 43.7 % and 75 putative open reading frames. The genome was grouped into four modules, including phage structure, DNA packaging, DNA replication and regulation, and some additional functions. Further genomic comparison of the phage PVA1 with other known phages showed no significant similarities. Genes related to virulence and lysogeny were not detected in the phage genome. Our results suggest that phage PVA1 may be classified as a new Vibrio phage. We believe that these phage genomic sequence data will provide useful basic information for further molecular research on this Vibrio phage and its host as well for determining its infection/interaction mechanisms.

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Acknowledgments

Support for this work was provided by the National Natural Science Foundation of China (No. 31001053), the National High-Tech R&D Program of China (863 Program) (No. 2006AA10Z412) and the Natural Science Foundation of Liaoning Province (No. 201202034).

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Authors and Affiliations

  1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, China

    Jiancheng Zhang, Zhenhui Cao, Yongping Xu, Xiaoyu Li, Lili Wang, Fang Cao, Zhen Li & Liji Jin

  2. Ministry of Education Center for Food Safety of Animal Origin, Dalian, 116600, China

    Jiancheng Zhang, Yongping Xu, Huaqiang Li, Feifei Wu, Lili Wang & Liji Jin

  3. Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China

    Zhenhui Cao

  4. Department of Biology and Chemical Engineering, Shaoyang University, Shaoyang, 422100, China

    Huaqiang Li & Feifei Wu

  5. Dalian SEM Bio-Engineering Technology Co. Ltd., Dalian, 116620, China

    Shuying Li

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  1. Jiancheng Zhang
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  2. Zhenhui Cao
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  11. Liji Jin
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Correspondence to Liji Jin.

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Zhang, J., Cao, Z., Xu, Y. et al. Complete genomic sequence of the Vibrio alginolyticus lytic bacteriophage PVA1. Arch Virol 159, 3447–3451 (2014). https://doi.org/10.1007/s00705-014-2207-z

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  • DOI: https://doi.org/10.1007/s00705-014-2207-z

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