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Complete genomic sequence of bacteriophage P23: a novel Vibrio phage isolated from the Yellow Sea, China

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Abstract

A novel Vibrio phage, P23, belonging to the family Siphoviridae was isolated from the surface water of the Yellow Sea, China. The complete genome of this phage was determined. A one-step growth curve showed that the latent period was approximately 30 min, the burst size was 24 PFU/cell, and the rise period was 20 min. The phage is host specific and is stable over a range of pH (5–10) and temperatures (4–65 °C). Transmission electron microscopy showed that phage P23 can be categorized into the Siphoviridae family, with an icosahedral head of 60 nm and a long noncontractile tail of 144 nm. The genome consisted of a linear, double-stranded 40.063 kb DNA molecule with 42.5% G+C content and 72 putative open reading frames (ORFs) without tRNA. The predicted ORFs were classified into six functional groups, including DNA replication, regulation and nucleotide metabolism, transcription, phage packaging, phage structure, lysis, and hypothetical proteins. The Vibrio phage P23 genome is a new marine Siphoviridae-family phage genome that provides basic information for further molecular research on interaction mechanisms between bacteriophages and their hosts.

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Acknowledgements

The research was funded by the Marine Scientific and Technological Innovation Project Financially supported by the Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0104-4, 2018SDKJ0406-6, and 2016ASKJ14), the National Natural Science Foundation of China (41676178, 31500339, and 41076088), the National Key Research and Development Program of China (2017YFA0603200 and 2018YFC1406704), the State Oceanic Administration People’s republic of China (GASI-02-PAC-ST-MSwin, GASI-02-PAC-ST-MSaut), and the Fundamental Research Funds for the Central University of Ocean University of China (201812002, 201762017, and 201562018).

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, People’s Republic of China

    Yundan Liu, Min Wang, Qi Wang, Xinran Zhang, Yuye Han, Meiwen Wang, Tong Jiang, Hongbing Shao & Yong Jiang

  2. Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, People’s Republic of China

    Min Wang, Hongbing Shao & Yong Jiang

  3. Qing Dao Municipal Hospital, Qingdao, People’s Republic of China

    Lei Zhao

  4. Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao, People’s Republic of China

    Min Wang & Yong Jiang

  5. Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, Australia

    Andrew McMinn

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  1. Yundan Liu
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  2. Lei Zhao
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  3. Min Wang
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  4. Qi Wang
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Contributions

Hongbing Shao, Yong Jiang, Tong Jiang, Meiwen Wang, Yuye Han, and Min Wang conceived and designed the experiments and critically evaluated the manuscript. Xinran Zhang and Qi Wang conducted the sequencing experiments. Lei Zhao isolated and identified the phage and conducted the biological characterization experiments. Andrew McMinn helped to edit the language of manuscript. Yundan Liu was responsible for the data and sequence analyses and wrote the manuscript.

Corresponding author

Correspondence to Min Wang.

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Liu, Y., Zhao, L., Wang, M. et al. Complete genomic sequence of bacteriophage P23: a novel Vibrio phage isolated from the Yellow Sea, China. Virus Genes 55, 834–842 (2019). https://doi.org/10.1007/s11262-019-01699-3

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