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Mixed infection of Sida jamaicensis in Jamaica reveals the presence of three recombinant begomovirus DNA A components

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Abstract

Begomoviruses impose serious constraints on agriculture throughout the temperate, tropical and subtropical regions. Previously, we characterised a sida golden yellow vein virus isolate, SiGYVV-[JM:Lig2:08] (HQ009519-20) from a symptomatic Sida jamaicensis plant. With the aim of establishing whether it was hosting a mixed infection that could facilitate recombination, PCR-RFLP was done on DNA extracted from this plant, and the results suggested the presence of two additional genetically distinct DNA-A molecules. Sequence analysis of these two DNA-A molecules (relying on BLAST searches and the CLUSTAL V algorithm within the DNASTAR MegAlign module) revealed that they belonged to novel species, and we have tentatively named these viruses sida golden mosaic Braco virus-[Jamaica:Liguanea:2008] and sida golden mosaic Liguanea virus-[Jamaica:1:2008]. Using RDP4 (recombination detection program), we determined that all three viruses were recombinant, with bases ~10 to ~440 of both SiGMLigV-[JM:Lig:08] and SiGYVV-[JM:Lig2:08] having been derived from a relative of SiGMBV-[JM:Lig:08] (P < 2.070 × 10−7 for all seven of the recombination detection methods). SiGMBV-[JM:Lig:08] was itself a product of recombination, deriving bases ~490–1195 from a virus that was ~92 % similar to malvastrum yellow mosaic Helshire virus. Phylogenetically, these DNA-A components are most closely related to those of malvaceous weed-infecting begomoviruses from Jamaica, Cuba, Florida and Mexico. The SiGMBV DNA-A was able to elicit symptomatic infection in N. benthamiana.

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Acknowledgments

Funding for this work was provided by the School of Graduate Studies and Research and the Principal’s New Initiative Fund, University of the West Indies, and by the Department of Plant Pathology, University of California Davis.

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Authors and Affiliations

  1. The Biotechnology Centre, The University of the West Indies, 2 St. John’s Close, Kgn 7, Mona, Jamaica

    Cheryl Stewart, André Graham & Marcia Roye

  2. The Department of Plant Pathology, The University of California Davis, 1 Shields Avenue, Davis, CA, 95616, USA

    Tatsuya Kon, Maria Rojas & Robert Gilbertson

  3. Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, 7925, Republic of South Africa

    Darren Martin

Authors
  1. Cheryl Stewart
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  2. Tatsuya Kon
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  3. Maria Rojas
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  4. André Graham
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  5. Darren Martin
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  6. Robert Gilbertson
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  7. Marcia Roye
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Correspondence to Cheryl Stewart.

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Stewart, C., Kon, T., Rojas, M. et al. Mixed infection of Sida jamaicensis in Jamaica reveals the presence of three recombinant begomovirus DNA A components. Arch Virol 159, 2509–2512 (2014). https://doi.org/10.1007/s00705-014-2063-x

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  • DOI: https://doi.org/10.1007/s00705-014-2063-x

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